姜黄素衍生物B06对2型糖尿病大鼠肝脏的保护作用  

Effect of curcumin derivative B06 on liver from type 2 diabetic rats

作　　者：王玲[1] 徐梦菲 刘曦[2] 王维维[3] 陈三妹[4] 程锦国[2] 陈国荣[1] 

机构地区：[1]温州医科大学附属第一医院病理科,浙江温州325015 [2]温州医科大学附属第二医院,浙江温州325015 [3]浙江大学附属第二医院病理科,浙江杭州310000 [4]绍兴文理学院医学院,浙江绍兴312000

出　　处：《中国病理生理杂志》2014年第2期328-332,338,共6页Chinese Journal of Pathophysiology

基　　金：浙江省公益性技术应用研究计划项目(No.2011c23123);温州市高层次人才创新技术项目

摘　　要：目的:研究姜黄素衍生物B06对2型糖尿病大鼠肝脏的保护作用及机制。方法:雄性SD大鼠35只,随机均分成5组:正常对照组、高脂组、高脂治疗组、糖尿病组和糖尿病治疗组。采用高脂饮食加链脲佐菌素诱导2型糖尿病大鼠模型。高脂治疗组及糖尿病治疗组用0.2 mg·kg-1·d-1B06灌胃8周。治疗结束后用光镜和透射电镜观察大鼠肝组织的形态学改变,并用Western blotting方法检测肝脏AMP活化蛋白激酶α(AMPKα)和磷酸化AMPKα(p-AMPKα)蛋白的表达。结果:高脂组及糖尿病组大鼠肝脏显示肝细胞脂肪变性、坏死,炎症细胞浸润,纤维组织增生,2型糖尿病大鼠和高脂血症大鼠肝脏组织p-AMPKα表达降低;经B06干预后,糖尿病大鼠和高脂血症大鼠肝脏的形态学损害明显得到改善,且肝脏组织p-AMPKα的表达水平升高(P<0.05)。结论:B06对2型糖尿病大鼠的肝脏具有保护作用,可能与其上调肝脏p-AMPKα蛋白表达有关。AIM ： To observe the protective effect of curcumin derivative B06 on the liver from the rats with hy- perlipidemia and type 2 diabetes mellitus. METHODS ： Male Sprague-Dawley rats （ n = 35 ） were divided randomly into 5 groups： normal control group, high-fat group, high-fat ＋ B06-treated group, diabetic group and diabetic ＋ B06-treated group. After fed with a high-fat diet for 4 weeks, the rats in the later 2 groups were injected with streptozotocin intraperito- neally to induce type 2 diabetes mellitus. The rats in B06-treated groups were given B06 by gavage at a dose of 0.2 mg · kg ^- 1·d^ - 1 for 8 weeks. After the treatment, the morphology of the liver was observed under light and transmission electron microscopes. The protein expression of AMP-activated protein kinase α （AMPKα） and phosphorylated AMPK α （p- AMPKα） was detected by Western blotting. RESULTS： Fatty degeneration, hepatocellular necrosis, inflammatory cell in- filtration and hyperplasia of fibrous tissue were observed in the liver from the rats in high-fat group and diabetic group, and were relieved after B06 treatment. The protein expression of p-AMPKα was decreased in the liver of the rats in diabetic group and high-fat group, and it was increased in the liver of the high-fat and diabetic rats in B06-treated group. CON- CLUSION： Cureumin derivative B06 exerts a protective effect on the liver in type 2 diabetic rats, and the increased ex- pression of p-AMPKα may be involved in the mechanism of protection.

关 键 词：糖尿病 高脂血症 姜黄素衍生物B06 肝 AMP活化蛋白激酶仪 大鼠 

分 类 号：R587.1[医药卫生—内分泌]