新生大鼠心肌成纤维细胞培养方法的比较  

Comparison of two methods for culturing neonatal rat cardiac fibroblasts

作　　者：孙刚[1,2] 薛睿聪[1,2] 刘晨[1,2] 董吁钢[1,2] 

机构地区：[1]中山大学附属第一医院心血管医学部,广东广州510080 [2]中山大学卫生部辅助循环重点实验室,广东广州510080

出　　处：《中国病理生理杂志》2014年第2期380-384,共5页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目(No.S2011040003614);广东省科技计划(No.B2011071)

摘　　要：目的:建立并比较新生大鼠心肌成纤维细胞的培养方法。方法:分别通过胶原酶+胰酶消化法和胰酶消化法对新生大鼠心肌组织进行消化,利用贴壁时间差分离心肌细胞和心肌成纤维细胞,然后对收集的心肌成纤维细胞进行形态、纯度及对药物反应方面的检测。结果:在细胞形态和纯度方面,2种方法收集的心肌成纤维细胞没有明显差异,但用胶原酶+胰酶消化法可以获得更多数量的心肌细胞;在药物反应方面,采用胰酶消化法获得的心肌成纤维细胞比胶原酶+胰酶消化法具有更强的增殖能力。结论:采用胶原酶+胰酶消化法和胰酶消化法可以获得相同形态和纯度的心肌成纤维细胞,但是采用胰酶消化法获得的心肌成纤维细胞对药物具有更强的增殖反应能力。AIM： To establish and compare the methods for culturing neonatal rat cardiac fibroblasts culture. METHODS ： Neonatal rat hearts were isolated by collagenase ＋ trypsin or trypsin digestion. The cardiomyocytes and cardi- ac fibroblasts were isolated by different attachment techniques. The cellular morphology, purity and reactions to the reagent were tested. RESULTS ： For morphology and purity, no difference between the 2 methods was observed, though more myo- cytes were harvested by the method of cotlagenase ＋ trypsin digestion. For the cellular responses to reagent, the cardiac fi- broblasts harvested with trypsin digestion had more potent proliferative ability than those with collagenase ＋ trypsin diges- tion. CONCLUSION ： There is no difference of cellular morphology and purity in the cardiac fibroblasts isolated by colla- genase ＋ trypsin digestion and trypsin digestion, but the fibroblasts with trypsin digestion have more potent proliferative abil- ity.

关 键 词：心肌成纤维细胞 胶原酶 胰酶 

分 类 号：Q253[生物学—细胞生物学]