孤独症大鼠前额叶皮质神经元凋亡相关蛋白的表达  

Expression of the apoptosis-related protein in prefrontal cortex in a rat model of autism

作　　者：文敏[1] 赵晶 鲍星星[3] 邓成敏[4] 童雪涛[3] 

机构地区：[1]贵阳医学院人体解剖学教研室,贵阳550004 [2]贵阳市第二人民医院神经内科,贵阳550004 [3]贵阳医学院附属医院儿科,贵阳550004 [4]贵阳医学院生物技术教研室,贵阳550004

出　　处：《山东大学学报（医学版）》2014年第2期48-51,共4页Journal of Shandong University:Health Sciences

基　　金：贵州市科技计划项目[筑科合同(2011103)18号];贵州省科技攻关计划课题[黔科合SY(2010)3082号]

摘　　要：目的观察孤独症大鼠前额叶皮质神经元凋亡相关蛋白Caspase-3和Bcl-2表达的变化，探讨细胞凋亡在孤独症发病中的作用。方法健康繁殖期Wistar雌鼠20只，体质量250～260g，随机选取10只在E12．5腹腔注射丙戊酸钠（VPA），其子代为孤独症模型组；其余10只在E12．5腹腔注射生理盐水，其子代为对照组。通过比较负向性实验、自梳理实验验证模型是否成功；Westemblotting方法对比对照组与孤独症模型组大鼠P42前额叶皮质凋亡相关蛋白Caspase-3和Bcl-2表达的变化。结果成功建立孤独症动物模型。与对照组比较，孤独症模型组大鼠旋转调头时间显著延长（P〈0．05），理毛时间显著增加（P〈0．05）；P42前额叶皮质凋亡相关蛋白Caspase-3表达显著降低（P〈0．05），Bcl-2表达显著升高（P〈0．05）。结论孤独症大鼠P42前额叶皮质凋亡受到抑制，提示调节凋亡可能是一个潜在的孤独症治疗策略。Objective To investigate the change of apoptosis-related protein Caspase-3 and Bcl-2 and effect of apoptosis in the rat model of autism. Methods Ten female Wistar rats （250-260 g） out of 20 were selected randomly to receive a single intraperitoneal injection of sodium valproate （VPA） at the pregnancy day of 12.5, whose offspring were de- fined as the autism model group. The rest 10 female Wistar rats received the intraperitoneal injection of saline, whose offspring were defined as the control group. To confirm whether the animal model was successfully established, the negative geotaxis test and self-grooming test were compared between the autism model group and control group. West- ern blotting was used to detect the expressions of Caspase-3 and Bcl-2 in prefrontal cortex in the two groups. Results The animal model of autism was successfully established. Compared with the control group, the negative geotaxis time and the cumulative self-grooming time in the autism model group significantly increased （ P 〈 0.05 ） ; the expression of Caspase-3 decreased while Bcl-2 in prefrontal cortex incresed（ all P 〈 0.05） in the autism model group. Contusion The apoptosis is inhibited in prefrontal cortex of autism, which suggests that the modulation of apoptosis might be a potential therapeutic strategy for autism.

关 键 词：孤独症 Caspase一3 BCL-2 凋亡 

分 类 号：R729[医药卫生—儿科]