不同剂量氟苯尼考对罗非鱼血药浓度的影响及组织毒理学研究  

Blood concentration and histological toxicology of different dosages of florfenicol in GIFT Nile tilapia

作　　者：王伟利[1] 罗理[1] 姜兰[1] 常藕琴[1] 鞠晶[1,2] 肖贺[1,2] 谭爱萍[1] 邓玉婷[1] 赵飞[1] 

机构地区：[1]中国水产科学研究院珠江水产研究所农业部渔药创制重点实验室,广东广州510380 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《上海海洋大学学报》2014年第1期90-94,共5页Journal of Shanghai Ocean University

基　　金：公益性行业(农业)科研专项(201203085);广东省科技计划项目(2011B020307001)

摘　　要：为研究氟苯尼考在罗非鱼(Oreochromis niloticus)体内的吸收特征及组织毒理,在30℃实验条件下,分别按0、5、12、20、40、80、100 mg/kg的给药剂量,通过药饵单次饲喂实验罗非鱼氟苯尼考后,使用HPLC方法检测各剂量组的血药浓度,结果发现各剂量组10 h的血药浓度分别为0、1.49±0.46、2.66±0.62、5.08±0.75、10.60±2.34、13.74±2.87、14.42±0.49μg/mL(M±SD,n≥6,n为各浓度点数据量),表明随着给药剂量的增加,实验动物体内血药浓度也随之上升,但40 mg/kg剂量以上,其浓度增幅降低,提示药物利用率随着给药剂量的增加而降低。在相同试验条件下,按照0、12、20、40、100 mg/kg的给药剂量连续饲喂罗非鱼7 d以研究药物对罗非鱼的组织毒理特性,组织切片显示20 mg/kg以下剂量组未见明显病变,在高剂量组(40 mg/kg、100 mg/kg)则出现了剂量依赖的肝肾细胞损伤现象,并且该损伤在继续饲养7 d的情况下未见修复。建议生产上的给药剂量不高于20 mg/kg,以免造成机体组织损伤,利用率降低及环境污染等问题。Florfenicol（FFC）,a synthetic broad-spectrum antibiotic,with a structural analogue of thiamphenicol is active against many thiamphenicol-resistant strains. Due to its broad antibacterial spectrum and with no side effects of aplastic anemia, FFC has been widely used in livestock,poultry,and is also legally permissive to be used in aquaculture. To study the absorption characteristics of florfenicol in GIFT Nile tilapia, which was the biggest quantity export farming fish, the 10 hour blood samples were detected using high-performance liquid chromatography with fluorescence detection method after feed administration of 0,5,12,20,40,80,100 mg/kg of florfenicol at 30℃.Results showed：the concentration was （MeanStandard Deviation,n≥6） 0,1.490.46,2.660.62,5.080.75,10.602.34, 13.742.87 and 14.420.49 μg/mL respectively. The concentration increased with the increase of dosage at the same time in blood,while the increase amplitude decreased, which indicated the decreased utilization of drug to the increasing dosage. In the same experiment condition, 0 （control）, 12,20,40,100 mg/kg body weight of florfenicol a day were given to GIFT in feed for 7 consecutive days to study the histological toxicology of florfenicol.There were no mortalities or clinically observable changes found at any of the dose levels tested. Dose dependent ultrastructural damages were found in cells of kidney and liver at the dosages of 40 and 100 mg/kg,the damage could not be repaired after 7 days at the end of the test, while no obvious pathological changes were found at dosages under 20 mg/kg. We recommend the dosage of no more than 20 mg/kg, otherwise it may damage the function of liver and kidney,lower utiliztion and cause environmental pollution, etc.

关 键 词：氟苯尼考 罗非鱼 不同剂量 组织毒理 

分 类 号：S948[农业科学—水产养殖]