羟丁基壳聚糖CD_(133)^+抗体温敏膜对人脐静脉内皮细胞生长的影响  

Effect of hydroxybutyl- chitosan carries CD_(133)^+ anti-thermosensitive membrane on growth of human umbilical vein endothelial cells

作　　者：张学丽[1] 李健[1] 李丹[1] 刘相萍[1] 安毅[1] 

机构地区：[1]青岛大学医学院附属医院,山东青岛266000

出　　处：《山东医药》2014年第1期1-3,17,共4页Shandong Medical Journal

基　　金：国家自然基金面上项目(81071246);山东省自然基金面上项目(ZR2010HM081)

摘　　要：目的观察复合羟丁基壳聚糖纳米载体携载CD+133抗体温敏膜对人脐静脉内皮细胞(HUVEC)增殖、迁移及血液相容性的影响。方法将HUVEC接种于裸钴铬合金片膜(裸金属支架组)、雷帕霉素药物涂层钴铬合金片膜(雷帕霉素支架组)、复合羟丁基壳聚糖纳米载体携载CD+133抗体温敏膜钴铬合金片膜(抗温敏膜组)。培养24 h后,在倒置相差显微镜下观察细胞形态;以MTT比色法检测细胞增殖活性,划痕试验检测细胞的迁移能力,ELISA检测各组细胞上清液中内皮素-1(ET-1)、降钙素基因相关肽(CGRP)、前列环素代谢物6-酮-前列腺素F1α(PGF1α)水平。结果 HUVEC在抗温敏膜上生长良好,形态正常。与裸金属支架组、雷帕霉素支架组相比,在抗温敏膜组存活的HUVEC数目增高(P均<0.05)。HUVEC在抗温敏膜组上迁移率高于裸金属支架组、雷帕霉素支架组(P均<0.05)。与裸金属支架组、雷帕霉素支架组相比,抗温敏膜组细胞分泌的ET-1水平降低;CGRP、PGF1α水平升高(P均<0.05)。结论复合羟丁基壳聚糖纳米载体携载CD+133抗体温敏膜有助于HUVEC的增殖和迁移,对HUVEC的血液相容性良好。Objective To investigate the influence of hydroxybutyl-chitosan nanoparticles carries CD133＋ anti-thermosensitive membrane on the proliferation,migration and hemocompatibility of human umbilical vein endothelial cells （HUVECs）.Methods HUVECs were inoculated into bare cobalt-chromium alloy film （bare metal stent group）,sirolimus-eluting cobaltchromium alloy film （sirolimus stent group） and hydroxybutyl-chitosan nanoparticles carries CD133＋ anti-thermosensitive membrane cobalt-chromium alloy film （anti-thermosensitive membrane group）.After being cultured for 24 hours,the cell morphology was observed by inverted phase-contrast microscope.The cell proliferation was measured by MTT colorimeter.The ability of cell migration was detected by scratch tests.Enzyme linked immunosorbent assay （ELISA） was used to measure the levels of endothelin-1 （ET-1）,calcitonin gene-related peptide （CGRP） and prostacyclin metabolite 6-keto-prostaglandin F1α （PGF1 α） in all groups.Results HUVECs grew well in the anti-thermosensitive membrane group,with normal morphology.Compared withthe baremetal stent and sirolimus stent groups,the number of survived HUVECs in the anti-thermosensitive membrane group was higher （all P ＜0.05）.The migration ratio in the anti-thermosensitive membrane group was higher than that in the bare metal stent and sirolimus stent groups,respectively （all P ＜ 0.05）.Compared with bare metal stent and sirolimus stent groups,the concentration of ET-1 of anti-thermosensitive membrane group decreased,and increased in the CGRP and PGF1α levels （all P ＜0.05）.Conclusions Hydroxybutyl-chitosan nanoparticles carries CD133＋ anti-thermosensitive membrane cobalt-chromium alloy film can improvethe proliferation,migration and hemocompatibility of HUVECs.

关 键 词：人脐静脉内皮细胞 壳聚糖 CD133+ 内皮素-1 降钙素基因相关肽 前列环素代谢物6-酮-前列腺素F1α 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R541.4[医药卫生—基础医学]