一起山羊伪结核棒状杆菌感染的诊断  

Diagnosis of Corynebacterium pseudotuberculosis Infection in Goats

作　　者：曾泽[1] 韦雷飞 黄秀君[2] 张斌[1] 于学辉[1] 汤承[1] 

机构地区：[1]西南民族大学生命科学与技术学院,四川成都610041 [2]攀枝花市农林科学研究院,四川攀枝花617061

出　　处：《动物医学进展》2014年第3期131-133,共3页Progress In Veterinary Medicine

基　　金：国家星火计划项目(2012GA810001)

摘　　要：2013年7月,四川省攀枝花某羊场发生淋巴结脓肿,从发病羊脓肿脓汁中无菌采集5份病料,病料接种于含血清的TSA平板上可长成扁平、不透明、干燥、松脆、瓷白色、易推动的菌落。革兰染色、镜检,可见革兰阳性小杆菌。经过PCR扩增及测序分析确定分离所得5株细菌为伪结核棒状杆菌,由此可以确诊该羊场为伪结核棒状杆菌感染。药敏试验结果表明该菌对强力霉素、四环素、氯霉素等药物较敏感,可以选取这些药物对该养殖场羊进行治疗。In July 2013,the goats with lymphonodus abscess were outbroken in a farm of Panzhihua city of Sichuan province.In this study,the five samples were sterily collected from the abscesses of goats.By bacterioscopy,the applanate,opaque,dry,crisp,white and Gram-negative bacilliform bacillus colonies were found after culture on the TSA culture medium containing the serum.The PCR detection and sequencing confirmed that the bacteria were the Corynebacterium pseudotuberculosis.In addition,the drug sensitive test indicated that the bacteria were sensi-tive to doxycycline,tetracycline and chloramphenicol,which could provide a treatment for this disease in this farm.

关 键 词：伪结核棒状杆菌 聚合酶链反应 药敏试验 

分 类 号：S852.612[农业科学—基础兽医学]