不同日龄鸡眼结膜相关淋巴组织的形态学观察  

Morphology observation of conjunctiva-associated lymphoid tissue in chickens at different ages

作　　者：杨树宝[1] 金唯[1] 栾维民[1] 彭琪[1] 董红艳[1] 马金萍[1] 钱爱东[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《中国兽医杂志》2014年第1期30-31,I0005,共3页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金项目(30901065);吉林省科技发展计划项目(201205059);吉林省教育厅"十一五"科学技术研究项目(2009582)

摘　　要：(目的)本试验旨在观察不同日龄鸡眼结膜相关淋巴组织(conjunctiva-associate lymphoid tissue,CALT)的形态结构,从而了解其不同时期的免疫状态。(方法)选择不同日龄的雏鸡,每个时期取3只雏鸡的下眼睑结膜制成冰冻切片,片厚5μm,然后进行H.E.染色。(结果)结果发现,鸡CALT的黏膜上皮为复层扁平上皮,常见杯状细胞。固有层内的淋巴细胞随着日龄增长逐渐增多,21日龄时可观察到生发中心,与淋巴滤泡相关的上皮常缺乏杯状细胞,27日龄CALT的各种组织结构基本成熟。(结论)结果表明,鸡CALT的组织结构随着日龄增长逐渐发育成熟,并在27日龄时其免疫功能基本达到成熟水平。[Objective]The aim of this study was to investigate the histological structure of conjunctiva-associate lymphoid tissue （CALT） in chickens at different ages.[Method]With lower eyelids of chickens as tested materials, we have investigated the immune state in different period using HE stain. [Result]The mucosa epithelium of CALT was stratified squamous epithelium, goblet cells were among them. With the increasing age, there was a rising tendency in lymphocytes of lamina propria. The germinal centers appeared at 21-day-old, but the lymphoid follicle of CALT was lacking in goblet cells. It began to reach mature level at 27-day-old in terms of its histological structure. [Conclusion] The histological structure of CALT in chickens developed gradually to maturity with the increase of age, and the immune function reached the mature level basically at 27 -day-old.

关 键 词：鸡 眼结膜 淋巴细胞 形态结构 

分 类 号：S852.4[农业科学—基础兽医学]