高原脑水肿大鼠脑组织水通道蛋白-4表达的变化及意义  

Change of aquaporins-4 expression in brain tissues of rats with high altitude cerebral edema and its significance

作　　者：梁林[1] 杨术旺[2] 龚海英[3] 董化江[4] 

机构地区：[1]武警后勤学院科研部,天津300162 [2]武警后勤学院训练部研究生处,天津300162 [3]武警后勤学院预防医学系药物化学教研室,天津300162 [4]武警后勤学院临床医学系人体解剖学与组织胚胎学教研室,天津300162

出　　处：《新乡医学院学报》2014年第2期90-92,共3页Journal of Xinxiang Medical University

基　　金：国家十一五科技支撑计划课题(编号:2007BAK38B05);武警后勤学院院级科研基金项目(编号:WYM201011;WHTD201308-2)

摘　　要：目的探讨高原脑水肿大鼠脑组织水通道蛋白-4(AQP4)表达的变化及意义。方法 16只雄性Sprague Dawley大鼠随机分为缺氧水肿组和对照组,每组8只,制作大鼠高原缺氧损伤模型。检测2组大鼠脑组织水含量,并采用实时定量聚合酶链反应、免疫组织化学法、Western blot测定大鼠脑组织高原缺氧损伤24 h后AQP4 mRNA和蛋白表达变化。结果与对照组比较,缺氧水肿组大鼠脑组织水含量增加,水肿明显,AQP4 mRNA和蛋白表达量上调,差异均有统计学意义(P<0.05)。结论 AQP4在高原缺氧脑水肿的形成过程中发挥了重要作用,提示对AQP4的干预可减轻高原脑水肿损伤。Objective To study the change of aquaporins-4 （AQP4） expression in brain tissues of rats with high altitude cerebral edema and its significance. Methods Sixteen Spragne Dawley rats were divided into anoxic edema group and control group randomly, with 8 rats in each group. The model of high altitude cerebral edema was established. The moisture capacity of brain tissue was detected. The expression of AQP4 mRNA and protein were measured by reverse transcription polymerase chain reaction,immunohistochemistry and Western blot. Results Compared with control group, the moisture capacity of brain tissue increased,the edema was evident, and the expression of AQP4 mRNA and protein were up-regulated in anoxic edema group. The differences were significant（ P 〈 0.05 ）. Conclusion AQP4 might play an important role in the formation of high altitude cerebral edema,which indicates that interference of AQP4 may decrease the damage of high altitude cerebral edema.

关 键 词：水通道蛋白4 大鼠 高原缺氧 脑水肿 

分 类 号：R338[医药卫生—人体生理学]