正交试验法优选瘰疬膏水煎提取工艺  

Optimization of Water Extraction Technique for Luoli Pastes

作　　者：陈鹏英[1] 杨柳[1] 

机构地区：[1]武汉市中医医院药学部,武汉430014

出　　处：《中国药师》2014年第1期84-86,共3页China Pharmacist

基　　金：武汉市卫生局临床重点学科研究项目(编号:武卫[2005]294号)

摘　　要：目的:优选瘰疬膏最佳水煎煮提取工艺。方法:设计3因素3水平9组试验,考察加水量、煎煮时间、提取次数对干膏得率影响;同时选取方中君药玄参的主要有效成分哈巴俄苷作为含量考察指标,采用HPLC法测哈巴俄苷的含量。结果:加水量8倍、煎煮时间1 h,提取3次工艺为干膏得率最高,哈巴俄苷的含量最高。结论:根据试验的结果,同时考虑成本,拟定以加水量8倍,煎煮时间1 h,煎煮3次为最佳提取工艺。Objective： To investigate the water extraction technique for Luoli pastes. Methods ： According to the orthogonal exper- iment with three factors and three levels ,9 liquid samples by water extraction were prepared . The best water extraction technique was selected with the yield of extract and the content of harpagoside as the indices. Results ： The water extrac, tion with 8-fold water, 1 h boil- ing and 3 times extraction showed the highest extraction yield and harpagoside content. Conclusion： The optimal water extraction tech- nique is with 8-fold water,boiling lh and extracting 3 times.

关 键 词：瘰疬膏 正交试验 干膏得率 高效液相色谱法 哈巴俄苷 

分 类 号：R284.2[医药卫生—中药学]