甘草附子药对中总皂苷的含量测定  

Determination of Total Saponins in Drug Pair of Licorice and Aconite

作　　者：孙小玲[1] 何凡[2] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023 [2]辽宁中医药大学药学院

出　　处：《中国药师》2014年第1期93-95,共3页China Pharmacist

摘　　要：目的:建立药对甘草附子总皂苷的含量测定方法。方法:用香草醛-高氟酸试剂,在560 nm处有最大吸收,并时比色条件进行了优化。结果:该方法能较为准确的测定出药对中总皂苷的含量;总皂苷的浓度在0.034~0.102 mg·ml^(-1)范围内呈良好的线性关系(r=0.999 2),平均回收率100.2%,RSD=2.7%(n=6)。结论:该方法灵敏,准确可靠,重复性好,可作为药对甘草附子中总皂苷控制的方法。Objective： To establish a determination method for total saponins in the drug pair of licolice and aconite. Methods： A colorimetry method was used to determine the total saponins at 560 nm using vanilin-perchloric acid as the reagent. Results： The cali- bration range of glycyrrhizin acid was 0. 034-0. 102 mg·ml^-1 （r =0. 999 2）. The mean recovery was 100.2% and RSD was 2.7% （n = 6）. Conclusion： The method is simple, rapid, accurate and reproducible.

关 键 词：甘草 附子 总皂苷 药对 分光光度法 

分 类 号：R927.2[医药卫生—药学]