HPLC法测定染料木素丙烯酸树脂纳米粒的药物含量及包封率  

Determination of Content and Encapsulation Efficiency of Genistein-loaded Eudragit Nanoparticles by HPLC

作　　者：徐娜[1] 纪宏宇[2] 唐景玲[2,3] 吴琳华[2,3] 

机构地区：[1]哈尔滨医科大学附属第四医院药学部 [2]哈尔滨医科大学附属第二医院药学部,黑龙江省高校重点实验室 [3]哈尔滨医科大学药学院,哈尔滨150086

出　　处：《中国药师》2014年第2期204-206,共3页China Pharmacist

基　　金：国家自然科学基金青年科学基金项目(编号:81302705);黑龙江省青年科学基金项目(编号:QC2011C104)

摘　　要：目的：建立测定丙烯酸树脂纳米粒中染料木素含量的HPLC方法。方法：色谱柱：DiamonsilTM C18柱（250mm×4．6mm，5μm）；流动相：甲醇-水=85：15（v：v）；流速：1．0ml·min-1；柱温：25℃；检测波长：261nm；进样量：10斗l、结果：药物峰与辅料峰分离度良好，染料木素浓度在1．0～100．0μg·ml-1范围内线性关系良好（r=0．9998），平均回收率为99．85％（RSD=0．65％，n=9）。结论：本方法简便，快捷，准确度高，专属性强，适用于测定染料木素丙烯酸树脂纳米粒的含量及包封率。Objective： To establish an HPLC method for the determination of genistein in genistein-loaded Eudragit nanoparticles. Methods： The HPLC analysis was carried out on a DiamonsilTM C18 （250mm×4.6mm,5μm）column with the mobile phase of methanol-water （85： 15） at the flow rate of 1.0 ml ·min-1. The detection wavelength was set at 261nm and the column temperature was 25℃. Results： A good separation was displayed between the drug peak and the excipient peaks. An excellent linearity range for genistein was obtained from 1.0 μg·ml-1 to 100.0 μg·ml-1 （ r = 0.999 8 ）. The recovery of genistein was 99.85% （ RSD = 0.65% ,n = 9）. Conclusion： The method is simple, rapid, accurate and specific, which is suitable for the determination of content and encapsulation efficiency of genistein-loaded nanoparticles.

关 键 词：染料木素 丙烯酸树脂 纳米粒 高效液相色谱 含量 包封率 

分 类 号：R944.9[医药卫生—药剂学] R927.2[医药卫生—药学]