康视明合剂质量标准的提高研究  

Improved Quality Standard for Kangshiming Mixtures

作　　者：徐玥[1] 蔡烽[1] 张钦[1] 洪婷[1] 徐燕丰[1] 

机构地区：[1]上海市中医医院,上海200071

出　　处：《中国药师》2014年第2期218-220,共3页China Pharmacist

基　　金：上海市卫生局中药新药及院内制剂研发项目(编号:2011ZJ003)

摘　　要：目的：制定康视明合剂质量控制方法。方法：采用薄层色谱法（TLC）鉴别处方中的当归、黄柏、黄芪；采用高效液相色谱法（HPLC）对处方中的葛根素及芍药苷含量进行定量测定。结果：TLC法能定性检出当归、黄柏、黄芪，斑点清晰，且阴性对照无干扰。定量分析中葛根素和芍药苷分别在1．64～49．20μg·ml-1（r=0．9999）和4．22～63．30μg·ml-1（r=0．9996）范围内与峰面积呈良好线性，葛根素平均回收率为99．63％，RSD=2．14％（n=9）；芍药苷平均回收率为99．05％，RSD=2．70％（n=9）。结论：本方法准确、可靠、专属性强，可用于康视明合剂的质量控制。Objective： To improve the quality standard for Kangshiming nfixtures. Methods： The identification of Angelicae Sinensis Radix, Astragali Radix and Phellodendri Chinensis Cortex was carried out by TLC. The contents of paerarin and paeoniflorin in the preparations were determined by HPLC. Results： The spots displayed in TLC were clear without interference from the negative control. The linear range for puerarin was 1. 64-49.20 μg·ml-1 （ r = 0. 999 9） and 4.22-63.30 μg·ml-1 （ r = 0. 999 6 ） for paeoniflorin. The average recovery was 99.63 % （ RSD = 2.14% , n = 9 ） and 99.05 % （ RSD = 2.70% , n = 9 ） for puerarin and paeoniflorin, respectively. Conclusion： The method is accurate, reliable and specific＇,, and can be used in the quality control of Kangshiming mixtures.

关 键 词：康视明合剂 薄层色谱法 高效液相色谱法 质量标准 

分 类 号：TQ460.72[医药卫生—药物分析学]