蛭芪康胶囊的质量控制研究  

Study on Quality Control of Zhiqikang Capsules

作　　者：廖欣[1] 汪玥[1] 钱文慧[1] 黄鲁[1] 苏华[1] 任海祥[1] 

机构地区：[1]南京军区南京总医院制剂科,南京210002

出　　处：《中国药师》2014年第2期221-224,共4页China Pharmacist

摘　　要：目的：建立蛭芪康胶囊的质量控制方法。方法：采用薄层色谱法对制剂中的天麻蜜环菌粉、大黄、黄芪进行定性鉴别。采用3，5-二硝基水杨酸光度法（DNS）测定制剂多糖含量，采用Folin-酚法（Lowry法）测定制剂肽含量。结果：定性鉴别检出制剂中的天麻蜜环茵粉、大黄、黄芪；DNS法测定结果，多糖在6．412—32．060μg·ml-1的范围内呈良好的线性关系（r=0．9995），平均回收率为95．86％，RSD为0．86％（n=6）；Folin-酚法测定结果，肽在0．0597～0．2984mg·ml-1范围内呈良好的线性关系r=0．9990），平均回收率为100．3％，RSD为1．88％（n=6）。结论：本方法简便、准确，可作为该制剂的质控方法。Objective ： To establish the quality control of Zhiqikang capsules. Methods ： TLC was used to identify Gastrodia tuder halimasch, rhubarb and Astragalus mongholicus in the preparations. A speetrophotometry＇method with 3, 5-dinitrosalicylic acid （DNS） was used to measure the polysaceharide content in Zhiqikang capsules. A spectrophotometry method with Forint phenol method （Lowry） was used to measure the peptide content in the capsules. Results： The linear range of polysaecharide was obtained between 6. 412 and 32. 060μg·ml-1（r = 0. 999 5）, the average recovery was 95.86% and RSD was 0.86%. The linear range of peptide was ob- tained between 0.059 7 and 0.298 4 mg·ml-1 （r =0.999 0）, the average recovery was 100.3% and RSD was 1.88% （n =6）. Conclusion： The assay method is simple and accurate in the quality control of the preparations.

关 键 词：天麻蜜环菌粉 薄层鉴别 多糖 3 5-二硝基水杨酸光度法 肽 Folin-酚法 含量测定 

分 类 号：TQ460.72[医药卫生—药物分析学]