先天性人巨细胞病毒感染对乙肝疫苗免疫效果的影响  

Influence of congenital human cytomegalovirus infection on hepatitis B vaccine's immune effect

作　　者：韩启军[1] 温天莲[2] 沈国红[2] 管长丽[2] 窦冬冬[2] 

机构地区：[1] 潍坊医学院 山东潍坊 261053 [2] 山东大学附属济南市中心医院 山东济南 250013

出　　处：《临床儿科杂志》2014年第2期167-169,共3页Journal of Clinical Pediatrics

摘　　要：目的探讨先天性人巨细胞病毒(HCMV)感染对乙型肝炎(乙肝)疫苗免疫效果的影响。方法孕鼠腹腔注射HCMV悬液,以其子鼠为先天性HCMV感染模型,以健康大鼠的子鼠作为对照组。各组子鼠分别于生后1、3、5周接种乙肝疫苗,并于3、5、7、11周心脏采血,应用酶联免疫分析法检测各组子鼠血清乙肝表面抗体(HBsAb)滴度。结果随接种次数增加,两组子鼠血清HBsAb滴度均呈逐渐增加趋势,接种完成后随时间延长又有不同程度下降,两组子鼠血清HBsAb滴度随时间变化的差异均有统计学意义(P均<0.001)。在各个时间点(3、5、7、11周),先天性HCMV感染组子鼠的血清HBsAb滴度均低于对照组,差异有统计学意义(P均<0.01)。结论先天性HCMV感染可减弱乙肝疫苗免疫效果。Objectives To investigate the immune effect of hepatitis B vaccine under the influence of congenital human cytomegalovirus （HCMV） infection. Methods The newborn rat model of congenital HCMV infection was developed by intra-peritoneally inoculating pregnant rat with HCMV suspension,while the offsprings of healthy rats were used as the control group. Offspring rats in all groups were inoculated with hepatitis B vaccine in the postnatal 1st, 3rd and 5th week and were taken blood from hearts separately in 3rd, 5th, 7th and 11th week. Antibody to Hepatitis B surface antigen （HBsAb） titer in all groups was de-tected by ELISA method. Results The serum HBsAb titer in both groups all showed a trend of increasing gradually with added vaccinating times and decreased differently with time extending after completed vaccinations. Differences among changes of HBsAb titer along with prolonged time in each group were all statistically significant （P＆lt;0.001）. At all time points （3rd, 5th, 7th, 11th week）, the titer of serum HBsAb in congenital HCMV infection group was lower than that in the control group respectively, and there were statistically significant differences （P＆lt;0.01）. Conclusions Congenital HCMV infection could weaken the im-mune effect of hepatitis B vaccine.

关 键 词：人巨细胞病毒 先天性感染 乙肝疫苗 免疫效果 大鼠 

分 类 号：R392.1[医药卫生—免疫学]