CNP/NPR-B效应轴与NPR-C/NEP清除途径在大鼠梗阻肾脏的表达  

Expression of CNP/NPR-B axis and NPR-C/NEP elimination pathways in rats with unilateral ureteral obstruction

作　　者：赵雪奇[1] 汪婧 孔海波[1] 胡波[1] 胡鹏[1] 

机构地区：[1] 安徽医科大学第一附属医院儿科 安徽合肥 230022 [2] 南京儿童医院 江苏南京 210000

出　　处：《临床儿科杂志》2014年第2期170-177,共8页Journal of Clinical Pediatrics

基　　金：国家自然科学基金资助项目(No.81000306)

摘　　要：目的研究C-型利钠肽(CNP)/B-型利钠肽受体(NPR-B)效应轴与C-型利钠肽受体(NPR-C)/中性肽链内切酶(NEP)清除途径在单侧输尿管梗阻(UUO)大鼠肾脏的表达情况。方法原位杂交、荧光定量PCR、免疫组化和Western blot检测UUO大鼠术后24 h、72 h、1周、2周、3周、1个月、2个月和3个月时肾脏CNP、NPR-B、NPR-C、NEP及IV型胶原mRNA和蛋白的表达情况。结果 CNP主要表达于肾小管上皮细胞,在梗阻后即刻显著增加,随疾病进展增幅逐渐衰减。CNP高表达主要归因于UUO早期NPR-B表达上调。结论 NEP和NPR参与肾小管间质纤维化病程中CNP表达调控;NPR-C和NEP表达渐增是导致CNP增幅降低的可能原因。Objectives To study the expression features of C-type natriuretic peptide （CNP）/natriuretic peptide receptor-B （NPR-B） axis and two parallel elimination pathways, natriuretic peptide receptor-C （NPR-C） and neutral endopeptidase （NEP） in unilateral ureteral obstruction （UUO） rats. Methods CNP, NPR-B, NPR-C, NEP, Col-IV and type IV collagen （Col-IV） mRNA and proteins were determined by in situ hybridization, real-time PCR, immunohistochemistry and western blot in UUO rats at 24h, 72h, 1w, 2w, 3w, 1m, 2m and 3m. Results CNP expression tended to be higher immediately after ligation and de-clined along with the progression of disease, occurring predominantly in tubular epithelial cells. A high-level CNP may attribute to the elevated expression of NPR-B in the early phase of UUO. Conclusions NEP and NPR participate in the regulation of CNP expression in tubulointerstitial fibrosis. The gradual increased expression of NPR-C and NEP may cause the subsequent de-cline of CNP.

关 键 词：C-型利钠肽 利钠肽受体 中性肽链内切酶 肾小管间质纤维化 单侧输尿管梗阻 

分 类 号：R693.2[医药卫生—泌尿科学]