Ancient DNA sequences from Coelodonta antiquitatis in China reveal its divergence and phylogeny  

作　　者：YUAN JunXia SHENG GuiLian HOU XinDong SHUANG XiaoYan YI Jian YANG Hong LAI XuLong 

机构地区：[1]State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences [2]Faculty of Material Science and Chemistry, China University of Geosciences [3]Department of Science and Technology, Bryant University, 1150 Douglas Pike Smithfield

出　　处：《Science China Earth Sciences》2014年第3期388-396,共9页中国科学（地球科学英文版）

基　　金：Yang Hong was supported by a short term Guest Professor through the sponsorship of China University of Geosciences in Wuhan;supported by National Basic Research Program of China(Grant No.2011CB808800);National Natural Science Foundation of China(Grant Nos.40672006,40902008);"111"Project of Chinese State Administration of Foreign Experts Affairs(Grant No.B08030)

摘　　要：Ancient DNA data have supported a sister relationship between woolly rhinoceros and extant Sumatran rhinoceros.This relationship has been used to explore the divergent times for the woolly rhinoceros from their relatives.Complete and partial ancient DNA sequences of the mitochondrial cytochrome b(cyt b)gene were retrieved from bones of the late Pleistocene Coelodonta antiquitatis excavated from northern and northeastern China.The newly obtained sequences together with the European and northern Asian Coelodonta antiquitatis sequences from GenBank were used to estimate the evolutionary divergence time.Phylogenetic analyses showed the exchange of genetic information between the Chinese individuals and Coelodonta antiquitatis of north Asia,which also indicated a more recent evolutionary timescale(3.8–4.7 Ma)than previous molecular estimations(17.5–22.8 or 21–26 Ma)for woolly rhinoceros based on the fossil calibration of outgroups.This new timescale was more consistent with the fossil record of the earliest known genus Coelodonta.Ancient DNA data have supported a sister relationship between woolly rhinoceros and extant Sumatran rhinoceros.This relationship has been used to explore the divergent times for the woolly rhinoceros from their relatives.Complete and partial ancient DNA sequences of the mitochondrial cytochrome b（cyt b）gene were retrieved from bones of the late Pleistocene Coelodonta antiquitatis excavated from northern and northeastern China.The newly obtained sequences together with the European and northern Asian Coelodonta antiquitatis sequences from GenBank were used to estimate the evolutionary divergence time.Phylogenetic analyses showed the exchange of genetic information between the Chinese individuals and Coelodonta antiquitatis of north Asia,which also indicated a more recent evolutionary timescale（3.8–4.7 Ma）than previous molecular estimations（17.5–22.8 or 21–26 Ma）for woolly rhinoceros based on the fossil calibration of outgroups.This new timescale was more consistent with the fossil record of the earliest known genus Coelodonta.

关 键 词：Coelodonta antiquitatis cytochrome b gene divergence time PHYLOGENY 

分 类 号：Q915[天文地球—古生物学与地层学]