Lipid profiling of mammalian cells with in situ matrix-assisted laser desorption ionization-mass spectrometry  

作　　者：ZHANG YanDong LI HaiFang MA Yuan LIN JinMing 

机构地区：[1]Beijing Key Laboratory of Microanalytical Methods and Instrumentation [2]The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology [3]Department of Chemistry, Tsinghua University

出　　处：《Science China Chemistry》2014年第3期442-446,1-3,共5页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21227006,21275088)

摘　　要：The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological process.In this work,a mass spectrometry-based method for direct,in situ analysis of lipids in cells was reported.Mammalian cells were directly cultured on ITO-coated glass and then analyzed by matrix-assisted laser desorption ionization-mass spectrometry(MALDI-MS).The matrix application process was achieved by electrospray coating,which produced a homogenous layer of matrix crystal on the sample.The detection results and reproducibility are satisfactory.With this method,a profile of abundant membrane lipids is generated,which is characteristic of cell type.In conclusion,this in situ MALDI-MS cellular lipid analysis method provides a platform for sensitive and robust molecular profiling of mammalian cells.The analysis of cellular lipids and phospholipids has been of continuously increasing research interest due to the importance of these molecules in psychological process.In this work,a mass spectrometry-based method for direct,in situ analysis of lipids in cells was reported.Mammalian cells were directly cultured on ITO-coated glass and then analyzed by matrix-assisted laser desorption ionization-mass spectrometry（MALDI-MS）.The matrix application process was achieved by electrospray coating,which produced a homogenous layer of matrix crystal on the sample.The detection results and reproducibility are satisfactory.With this method,a profile of abundant membrane lipids is generated,which is characteristic of cell type.In conclusion,this in situ MALDI-MS cellular lipid analysis method provides a platform for sensitive and robust molecular profiling of mammalian cells.

关 键 词：lipid profiling MALDI-MS linear discriminative analysis 

分 类 号：Q2-3[生物学—细胞生物学]