Design of multiplex logic gates:combining regulation of DNA structure with logical calculation  

作　　者：TAO Jia ZHENG Jing LI JiShan ZHAO Peng LI JuanPing MA Cheng YI Mei YANG RongHua 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics [2]College of Chemistry and Chemical Engineering, Hunan University

出　　处：《Science China Chemistry》2014年第3期453-458,共6页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China(21005026 and 21135001);the National Basic Research Program of China(2011CB911000);the Foundation for Innovative Research Groups of NSFC(21221003)

摘　　要：In this paper,we proposed a facile and accurate way for controlling multiplex fluorescent logic gates through changing the exciting and the observing wavelengths.As proof-of-principle,a Pb2+-specific DNAzyme probe and a thymine(T)-rich DNA probe were introduced to a double-stranded(ds-)DNA.The addition style of the two ions served as the four inputs by changing the distance of the three fluorophores,6-carboxyfluorescein(FAM),ALEXA 532(ALEXA)and carboxytetramethylrhodamine(TAMRA),all of which were modified on the dsDNA probe.Compared with the previous methods,the present approach needed neither different inputs nor the change of sequence of the probe to achieve multiplex logic gates.Furthermore,the modularity of the strategy may allow it to be extended to other types of logic gates.In this paper,we proposed a facile and accurate way for controlling multiplex fluorescent logic gates through changing the exciting and the observing wavelengths.As proof-of-principle,a Pb2＋-specific DNAzyme probe and a thymine（T）-rich DNA probe were introduced to a double-stranded（ds-）DNA.The addition style of the two ions served as the four inputs by changing the distance of the three fluorophores,6-carboxyfluorescein（FAM）,ALEXA 532（ALEXA）and carboxytetramethylrhodamine（TAMRA）,all of which were modified on the dsDNA probe.Compared with the previous methods,the present approach needed neither different inputs nor the change of sequence of the probe to achieve multiplex logic gates.Furthermore,the modularity of the strategy may allow it to be extended to other types of logic gates.

关 键 词：logic gates FRET DNA mercury ions（Ⅱ） lead ions 

分 类 号：O629.7[理学—有机化学]