一株鼻病毒A22型的分离鉴定及其VP1基因遗传特征分析  

Isolation and identification of a rhinovirus A22 strain and analysis of genetic characters of its VP1 gene

作　　者：戴素萍[1] 潘玥[1] 邵聪文[1] 吉玛[1] 朱艳菊[1] 张云昆[1] 朱云[1] 马绍辉[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室,云南昆明650118

出　　处：《中国生物制品学杂志》2014年第2期177-179,184,共4页Chinese Journal of Biologicals

基　　金：云南省应用基础研究面上项目(2013FZ136)

摘　　要：目的分析2011年昆明市手足口病患儿粪便中分离获得的人鼻病毒（human rhinovirus,HRV）A22型分离株KM4/2011全长VP1基因的遗传特征。方法采用KMB17细胞对手足口病患儿粪便样本中筛查出的1株HRV样品进行病毒分离,应用RT-PCR法扩增病毒VP1基因,并进行测序,采用BLAST软件对所测定的节段序列进行数据库比对;采用Geneious basic 5.6.5软件进行核苷酸和氨基酸同源性比对;采用Mega 5.05软件将HRV A22及部分HRV A、B和C群全长VP1基因构建系统进化树。结果分离株为KM4/2011,经扩增、测序和序列拼接获得长度为867 bp的VP1基因;KM4/2011株与其他HRV分离株核苷酸和氨基酸的同源性分别为48.1%-91.0%和38.2%-97.6%,其中与HRV A22分离株的核苷酸和氨基酸同源性较高,分别为90.8%-91.0%和97.3%-97.6%;基因进化树分析显示,KM4/2011株与HRV A22基因型属于同一个进化分枝。结论 KM4/2011分离株为HRV A22型,存在地域差异。Objective To analyze the genetic characters of VP1 gene of human rhinovirus A22 KM4 / 2010 strain isolated from the feces of children with hand-foot-mouth disease in Kunming City,Yunnan Province,China in 2011. Methods KM4 / 2011 strain was isolated by using KMB17 cells,from which VP1 gene was amplified by RT-PCR and sequenced,then compared with those of other rhinovirus strains in GenBank by using BLAST and Mega 5. 05 software. Results The VP1 gene at a length of 867 bp was obtained by amplification by RT-PCR,sequencing and splicing,of which the homologies of nucleotides and amino acids were 48. 1% - 91. 0% and 38. 2% - 97. 6% to those of other representative rhinovirus strains,and 90. 8% - 91. 0% and 97. 3% - 97. 6% to those of rhinovirus A22 isolate,respectively. Phylogenetic analysis revealed that KM4 / 2011 strain and other rhinovirus A22 strains were segregated into one distinct cluster. Conclusion KM4 / 2011 strain was human rhinovirus A22,while regional difference of human rhinovirus A22 strain was observed.

关 键 词：鼻病毒A22型 VP1基因 序列分析 

分 类 号：R373.14[医药卫生—病原生物学] Q78[医药卫生—基础医学]