玉米穗上叶夹角和叶间距的QTL定位  

QTL Mapping for Leaf Angle and Leaf Space above Ear Position in Maize(Zea mays L.)

作　　者：刘正 余婷婷 梅秀鹏 陈淅宁 王国强[1] 王久光[1] 刘朝显[1] 王旭[1] 蔡一林[1] 

机构地区：[1]西南大学玉米研究所,重庆400715

出　　处：《农业生物技术学报》2014年第2期177-187,共11页Journal of Agricultural Biotechnology

基　　金：重庆市重大项目玉米育种新技术和新方法研究(No.CSTC2012ggC80003,No.CSTC2012ggC80006,No.CSTC2012ggC80007)

摘　　要：玉米(Zea mays L.)的叶夹角和叶间距是玉米株型的两个重要性状,不同株型通过影响群体冠层内的光分布状况和群体的光能利用而影响产量。为了解玉米叶夹角和叶间距的遗传机制,利用紧凑型玉米自交系CY5和平展型玉米自交系YL106组配构建的F2世代为作图群体,构建具有212个SSR标记的遗传连锁图谱,图谱总长度为1 153.39 cM,标记平均间距5.44 cM。利用144个F2∶3家系在3个环境下的表型值,结合已构建的遗传连锁图谱,采用完备区间作图法,对穗上叶夹角和穗上叶间距进行基因定位。结果表明,在单环境分析中,共检测出22个QTL,叶夹角和叶间距分别检测到10和12个QTL,其中西南大学玉米研究所歇马试验基地(简称XM)检测到9个,北碚试验基地(简称BB)检测到8个,合川试验基地(简称HC)检测到5个;在多环境联合分析中,共检测出11个QTL,分别位于1、3、5和7号染色体上,其中叶夹角检测到5个,位于1、3和5号染色体上,叶间距检测到6个,位于1和7号染色体上;在单环境和多环境中同时检测到的一致性QTL有3个,分别是穗上二叶夹角(qSecLA1a)、穗上三叶夹角(qThiLA1a)和穗上三叶间距(qThiLS7),其中在单环境中qSecLA1a位于1号染色体bnlg1803～bnlg1007,分别解释表型变异率26.99%和18.51%,qThiLA1a位于1号染色体bnlg1803～bnlg1007,分别解释表型变异率24.14%和22.00%,qThiLS7位于7号染色体bnlg1305～umc1787,分别解释表型变异率13.77%和9.96%;以上3个QTL在多环境联合分析中的贡献率分别为29.10%、31.86%和11.20%。因此,qSecLA1a、qThiLA1a和qThiLS7 3个位点在不同环境中可稳定表达。本研究结果可为玉米叶夹角和叶间距的遗传改良和分子标记辅助育种提供参考资料。The plant-type of maize（Zea mays L.） is characterized by the leaf angle and leaf spacing. And the yield is associated with the distribution of light within canopy and utilization of solar energy within groups, which are affected by the plant-types of maize. To analyse the genetic mechanism of leaf angle and leaf space above ear position in maize, a genetic linkage map composing of 212 simple sequence repeat markers（SSR） was constructed based on the F2 population derived from the cross between compact inbred line CY5 and horizontal-leaf type inbred line YL106. The map covered 1 153.39 cM with an average interval of 5.44 cM. By using the inclusive composite interval mapping method, QTLs for leaf angle and leaf space above ear position of maize were identified based on a field mapping population consisting of 144 F2∶3 families in three environments（XM, short for Xiema test site; BB, short for Beibei test site; HC, short for Hechuan test site）. Twenty-two QTLs were detected in single-environment analysis, ten for leaf angle and twelve for leaf space, including nine in XM, eight in BB and five in HC, respectively. Totally eleven QTLs were detected in joint-environment analysis, which were distributed on chromosomes 1, 3, 5 and 7. Five QTLs for leaf angle were detected, which were distributed on chromosomes 1, 3 and 5. Six QTLs for leaf space were detected, which were distributed on chromosomes 1 and 7. Three consistent QTLs including qSecLA1a, qThiLA1a and qThiLS7 were all detected in single-environment and joint-environment analysis. qSecLA1a between bnlg1803 and bnlg1007 explained 26.99% and 18.51% of the phenotypic variation, qThiLA1a between bnlg1803 and bnlg1007 explained 24.14% and 22.00% of the phenotypic variation, qThiLS7 between bnlg1305 and umc1787 explained 13.77% and 9.96% of the phenotypic variation, in single-environment analysis; while in joint-environment analysis explained 29.10%、31.86% and 11.20% of the phenotypic variation, respectively. Therefore it can be concluded that the three

关 键 词：玉米 叶夹角 叶间距 简单重复序列(SSR)标记 数量性状位点定位 

分 类 号：S828.2[农业科学—畜牧学]