水稻CMS-WA和CMS-DA育性恢复基因Rf3和Rf4的聚合效应分析  

Analysis of Pyramiding Effect of the Rf3 and Rf4 Genes on Fertility Restoration in Rice(Oryza sativa) with Wild Abortive and Dwarf Wild Abortive Cytoplasmic Male Sterility

作　　者：蔡健[1,2] 范海燕[1] 廖秋平[2] 张桂权[2] 

机构地区：[1]阜阳师范学院生命科学学院,阜阳236041 [2]华南农业大学广东省植物分子育种重点实验室,广州510642

出　　处：《农业生物技术学报》2014年第2期202-209,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金重点项目(No.30830074);国家星火计划项目(No.2013GA710018);安徽省高校省级自然科学基金重点项目(小麦T-CMS相关基因orf256/cox1的克隆与功能分析);广东省自然科学基金项目(No.9451064201003804)

摘　　要：为了选育水稻野败型(WA)和矮败型(DA)细胞质雄性不育(CMS)的较强恢复系和分析恢复基因Rf3和Rf4的聚合效应,本研究选取7个携带恢复基因Rf3(或者Rf4)的单片段代换系(indica)两两杂交,配制了10个杂交组合。利用分子标记辅助选择从这10个杂交组合的F2和F3群体中筛选鉴定6个携带基因型Rf3Rf3/Rf4Rf4的双片段聚合系和5个携带Rf3或者Rf4基因的次级单片段代换系。对于野败型和矮败型不育细胞质,这些双片段聚合系具有不同的恢复性。其中,双片段聚合系DSPL11-01/14-10P的基因型为Rf3-4Rf3-4/Rf4-4Rf4-4,表现出最强的恢复性,DSPL07-01/14-10P的基因型为Rf3-1Rf3-1/Rf4-1Rf4-1,表现出最弱的恢复性,其余双片段聚合系的恢复性介于二者之间。5个次级单片段代换系代换片段的总长度为93.5cM,分布在0.1～45.5 cM之间,平均长度为18.7 cM。利用恢复性最强的DSPL11-01/14-10P及其相应的单片段代换系分别与野败型不育系博白A和矮败型不育系协青早A杂交,通过考察F1杂种株的花粉育性以研究DSPL11-01/14-10P中的恢复基因Rf3和Rf4的聚合效应。结果表明,虽然DSPL11-01/14-10P与其相应的单片段代换系相比能够明显提高F1杂种株的花粉育性,但其差异没有达到显著水平,该聚合系聚合的恢复基因Rf3和Rf4之间没有互作效应。本研究结果将为水稻"三系"的选育和恢复基因的转移提供基础数据。Wild-abortive （WA） and dwarf wild abortive （DA） are two important cytoplasmic male sterility （CMS） types in rice（Oryza sativa）. CMS can be restored by nuclear restorer gene （Rf）. One or two dominant restorer alleles （Rf3 and/or Rf4） are usually suggested be responsible for the fertility of WA-CMS and DA-CMS. To develop the strong restorer lines for WA-CMS and DA-CMS and analyse the pyramiding effect of the Rf3 and Rf4 genes, seven indica chromosome double segments pyramiding lines （DSPLs）, carrying the genotype Rf3Rf3/Rf4Rf4, and five second single segment substitution lines （SSSSLs） with the Rf3 on chromosomes 1 （or Rf4 on chromosomes 10） gene were selected by marker-assisted selection （MAS） in the F2 and F3 populations generated from the ten crosses among the seven SSSLs which possess the Rf3 （or Rf4） gene on fertility restoration in rice with WA-CMS and DA-CMS in this study. There were wide differences in restoring abilities for WA-CMS and DA-CMS among the six DSPLs. Of which the restoration ability of the DSPL11-01/14-10P with the genotype Rf3-4Rf3-4/Rf4-4Rf4-4 was the strongest, the DSPL07-01/14-10P carrying the genotype Rf3-1Rf3-1/Rf4-1Rf4-1 appeared to be weakest restoring ability, and the restoration abilities of the other DSPLs ranged from DSPL11-01/14-10P to DSPL07-01/14-10P. The length of substituted chromosome segments in the five SSSSLs ranged from 0.1 cM to 45.5 cM with an average of 18.7 cM, and the total length of substituted chromosome segments from them was 93.5 cM. To reveal the pyramiding effect of the Rf genes, the DSPL11-01/14-10P and its corresponding SSSLs were crossed with a typical WA-CMS lines of Bobai A （BBA） and a typical DA-CMS line of XieqingzaoA （XQA）, respectively. In the F1 populations, the plants carrying the Rf3rf3/Rf4rf4 genotype were selected and their phenotyping for pollen was evaluated. The result showed that even if the restoration ability of the DSPL11-01/14-10P showed stronger than that of corresponding SSSLs, the diff

关 键 词：水稻 单片段代换系 双片段聚合系 次级单片段代换系 野败型 矮败型 

分 类 号：S634.4[农业科学—蔬菜学]