辣椒转录组SNP挖掘及多态性分析  

SNP Mining in Pepper Transcriptome and the Polymorphism Analysis

作　　者：刘峰[1,2] 谢玲玲[3] 弭宝彬[1] 欧阳娴[1] 茆振川[2] 邹学校[1] 谢丙炎[2] 

机构地区：[1]湖南农业科学院蔬菜研究所,长沙410125 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]湖南农业科学院西瓜甜瓜研究所,长沙410125

出　　处：《园艺学报》2014年第2期343-348,共6页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31101425);国家重点基础发展计划项目(2009CB119000);国家现代产业技术体系建设专项资金项目(CARS-25-B-01);公益性行业(农业)科研专项(2011303018)

摘　　要：利用SNP分析软件从辣椒(Capsicum annuum L.)251 068条Unigenes中筛选出18 159个SNP,其中有1 781个SNP位点被匹配在1 291个注释基因上,基因功能分类和代谢途径分析表明,其中有853个基因参与初生代谢(28.7%)、细胞代谢(17.3%)、生物合成过程(15.7%),另有125个(9.7%)基因序列参与新陈代谢途径,53条(4.1%)序列参与次生代谢产物合成途径,31条(2.4%)序列参与植物激素合成途径。EST-SNP序列中4 172条(22.9%)满足设计CAPS引物条件,为了验证EST-SNP正确性,并选取了15对CAPS引物对5份辣椒材料进行扩增,结果发现有8对(53.3%)引物表现出多态性。表明筛选出这些EST-SNP标记可作为辣椒基因分型、图谱构建等的候选分子标记。In this study, we investigated 251 068 unigenes from two transcriptome in pepper （Capsicum annuum L.） using SNP finding soft. In total, 18 159 SNP were identified from these SNP-containing unique ESTs. Among, 1 781 SNP located at 1 291 annotation genes, analysis of GO （Gene Ontology） showed that 853 ESTs were classified, among primary metabolic process （28.7%）, cellular metabolic process （ 17.3% ）, biosynthetic process （15.7%） being main. In KEGG map, 125 ESTs involved in taken part in metabolic pathways, and 53 participated in biosynthesis of second metabolites, and 31 involved in biosynthesis of plant hormones. Moreover, a total of 4 172 （22.9%） sequences were successfully designed primers of CAPS marker. Eight out of 15 primer pairs selected at random showed polymorphism among 5 different pepper varieties. The results indicated that those CAPS markers from EST-SNP will be more usable in map structure, analysis of genetic polymorphism in pepper.

关 键 词：辣椒 转录组 EST-SNP CAPS 

分 类 号：S641.3[农业科学—蔬菜学]