基于ISSR标记的红花檵木品种亲缘关系分析  

Genetic Relatives Analysis of 41 Loropetalum chinense var. rubrum Cultivars by ISSR Markers

作　　者：董海燕[1] 季孔庶[2] 侯伯鑫[3] 赵宏波[1] 

机构地区：[1]浙江农林大学风景园林与建筑学院,浙江临安311300 [2]南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京210037 [3]湖南省林业科学院,长沙410004

出　　处：《园艺学报》2014年第2期365-374,共10页Acta Horticulturae Sinica

基　　金：浙江省花卉新品种选育重大科技专项重点项目(2012C12909-16);江苏省高校优势学科建设工程项目(PAPD)

摘　　要：采用ISSR分子标记技术对红花榷木41个品种的遗传多样性进行了分析。从60条ISSR引物中筛选出14条引物分别对供试材料基因组DNA进行扩增，共获得203条清晰可辨谱带，其中多态性带148条，多态位点百分率为72．9％。应用NTSYS．pc（version2．10e）软件计算得到各品种间的Jaecard相似系数介于0．626～0．926之间。用UPGMA法将41个品种分成5个类群，支持叶色作为最高分类等级，其次应重视花瓣形状的利用，这两个性状最为稳定且能反映品种间系统演化；同时由于品种起源和选育的原因，品种分类还应适当考虑育种过程及其亲本的性状；花色、叶形、分枝性等变化没有明显规律，不宜作为太高的分类等级。ISSR-PCR was used to detect the genetic diversity and relationship of 41 varieties of Loropetalum chinense var. rubrum. ISSR fingerprinting amplified by 14 ISSR primers revealed a total number of 203 unambiguous bands, of which 148 ones were polymorphic and the polymorphism frequency was 72.9%. As analyzed by NTSYS-pc （version 2.10e）, the similarity coefficient between varieties ranged from 0.626 to 0.926. These 41 varieties ofL. chinense vat. rubrum were divided into five groups by UPGMA based on Jaccard coefficient. Leaf color could be the highest classification trait following with petal shape which reflected the phylogenetic evolution among different cultivars. Additionally, due to the origin and breeding of cultivars, the breeding procedure and the characteristics of parents should be considered in cultivar classification. The inheritance of flower color, leaf shape and habit of branching was not stable, so these traits were not suitable as higher classification standard.

关 键 词：红花檵木 ISSR 遗传多样性 亲缘关系 

分 类 号：S68[农业科学—观赏园艺]