子痫前期相关微小RNA-18b对人正常滋养细胞浸润能力影响的研究  

The Affect of Preeclampsia Related miR-18b on the Invasion Capacity of Human Normal Trophoblast Cell

作　　者：杨洋[1] 席瑞[1] 徐妍妍[1] 

机构地区：[1]西安医学院附属医院,陕西西安710077

出　　处：《实用妇产科杂志》2014年第2期132-135,共4页Journal of Practical Obstetrics and Gynecology

基　　金：陕西省教育厅科研项目(编号:12JK0770);西安医学院附属医院科研项目(编号:XYFY11-05)

摘　　要：目的:研究人类子痫前期相关微小RNA-18b(miR-18b)对人正常滋养细胞系(HTR-8/SVneo)细胞浸润能力的影响。方法:用脂质体包裹化学合成的miR-18b前体分子并转染人正常滋养细胞系,过表达与特异性抑制miR-18b。逆转录-聚合酶链反应技术(RT-PCR)检测miR-18b过表达与抑制效果;Tanswell小室技术检测转染后细胞浸润能力的变化。结果:RT-PCR结果显示,与未转染组(0.422±0.011)相比,miR-18b过表达组(0.910±0.040)及miR-18b抑制组(0.110±0.012)miR-18b的表达显著变化,差异均有统计学意义(P<0.05)。Tanswell小室检测结果显示,与未转染组及过表达组相比,抑制组细胞浸润能力有所下降,差异具有统计学意义(P<0.05);与未转染组相比,过表达组细胞浸润能力无明显改变,差异无统计学意义(P>0.05)。结论:抑制子痫前期相关miR-18b的表达可影响人正常滋养细胞的浸润能力,可能与子痫前期疾病的发生发展有关。Objective ：To investigate the effect of preeclampsia related miR-18b on the invasion capacity of normal trophoblast cell line（HTR-8/SVneo）. Methods：The mature type human miR-18b was synthesized chemically and enveloped in liposome. The HTR-8 cells which were transfected with synthesized miR-18b served as study group. HTR-8 cells which were transfected with empty vectors served as control group. The expression of mature type miR-18b was detected by RT-PCR. The invasive capacity of HTR-8 cells was checked by transwell assay. Results：The results of RT-PCR showed that there were significant differences on the expressions of miR-18b among miR-18b over-expressed group, miR-18b suppressed group and con- trol group（ P 〈 0.05）. The results of transwell assay showed that compared to miR-18b over-expressed group and control group, infiltration capacity of miR-18b suppressed group were significantly declined（ P 〈 0.05）. There was no significant difference on infiltration capacity between over-expressed group and control group （ P 〉 0.05）. Conclusions：Inhibiting the expression of miR-18b may affect the ability of trophoblastic in- vasion of human normal trophoblast cell,which may involved in the onset and develolpment of preeclampsia.

关 键 词：微小RNA 子痫前期 滋养细胞 浸润 

分 类 号：R714.244[医药卫生—妇产科学]