大鼠脑挫伤后NTE与COX-2表达随时间的变化  

The variation law of NTE and COX-2 with time in rat's brain which has been contused

作　　者：刘玉利[1] 田亚汀[1] 田晓菲[1] 王淑强[1] 史满金[1] 张国徽[1] 

机构地区：[1]河北北方学院法医系,河北张家口075000

出　　处：《中国法医学杂志》2014年第1期22-24,29,I0002,共5页Chinese Journal of Forensic Medicine

基　　金：张家口市科技攻关项目(0711046D-14);河北省卫生厅重点科技研究计划(08386)

摘　　要：目的观察大鼠脑挫伤后脑组织NTE和COX-2的表达变化,并评价其法医学的意义。方法健康SD大鼠48只,随机分成正常对照组、手术对照组、挫伤组(按挫伤后处死时间再分为1h、12h、1d、3d、7d、14d共6个组)每组6只。以自由落体撞击法制作脑挫裂伤模型;应用免疫组化方法检测COX-2和NTE在脑组织中的表达情况;用SPSS 13.0软件分析COX-2和NTE的表达情况与脑挫伤后存活时间的相关性。结果正常对照组和假手术组NTE和COX-2均呈低水平表达,脑挫伤组伤后NTE和COX-2表达逐渐增强,均于1d达到高峰,至14d时COX-2表达降至正常对照组水平,而NTE仍高于正常对照组水平;挫伤各组与正常对照组比较,除1h组灰度值无统计学差异(P>0.05)外,其余各组均有明显差异(P<0.01)。结论脑挫伤后脑组织NTE和COX-2的表达均呈现先增强后下降的趋势,其表达规律有望为脑挫伤诊断及损伤时间推断提供参考。Objective To observe the expression of NTE and COX-2 in rat＇s brain that has been contused, and to evaluate its forensic significance. Methods 48 rats were divided into three groups, control group（6）, sham operation group（6）, contusion group（36） which were subdivided into lh, 12h, ld, 3d, 7d and 14d groups （6 rats in each group）. After the model of brain contusion was set up in rats through freefaHing dart method, the expression of NTE and COX-2 will be observed using immunohistochemieal method. The correlation between the expression of them and the rat＇s living time after being contused will be analyzed with SPSS 13.0. Results The expression of NTE and COX-2 were in low level in both the control group and the sham operation control group. In contusion group the expression of them were gradually elevated, the peak of both NTE and COX-2 appear at ld, then weakened gradually. The expression of COX- 2 （positive cell number） have dropped to the normal control group at 14d, but NTE （grey scale value） have not. Compared with the normal control group, the 1 h group of NTE showed no obvious statistical significance （ P 〉 0. 05 ）, but other groups have obvious statistical significance （ P 〈 0. 01 ）. Conclusion The expression of NTE and COX-2 were both elevated after the brain has been contused, and then weakened. And it is expected to provide a reference for diagnosis of brain contusion and dating of wound.

关 键 词：法医病理学 脑挫伤 免疫组织化学 神经病靶酯酶 诱导型环氧合酶 

分 类 号：D919[医药卫生—法医学]