妊娠大鼠子宫胎盘缺血改良手术方法诱发妊娠高血压模型  

Gestational hypertension models induced by modified surgery for uteroplacental ischemia in pregnant rats

作　　者：姚蓓蓓 桂鸣 徐秀秀 杨国平 唐春平 黄峻 

机构地区：[1]南京医科大学第一附属医院心脏科,江苏南京210000 [2]南京医科大学第一附属医院动物实验中心,江苏南京210000

出　　处：《中华高血压杂志》2014年第1期74-78,共5页Chinese Journal of Hypertension

基　　金：江苏省科技厅国际技术合作项目(BZ2010040)

摘　　要：目的探讨子宫胎盘缺血(RUPP)改良手术方法制作妊娠高血压大鼠(Spragu-Dawley)模型的可行性。方法将30只SD妊娠大鼠随机分成3组:假手术组、RUPP经典手术组(于孕14d手术,在腹主动脉、双侧卵巢动脉置入银夹)、RUPP改良手术组(于孕14d手术,在腹主动脉、单侧卵巢动脉置入银夹),每组10只。结果孕19d时,与假手术组比较,RUPP经典手术、RUPP改良手术组收缩压[(157.4±11.8)、(161.4±6.3)比(117.0±9.4)mm Hg]、舒张压[(122.6±8.1)、(123.3±7.7)比(100.7±4.4)mm Hg]、心率[(398.4±10.1)、(400.5±13.1)比(383.9±11.5)次/min]、尿蛋白[(18.1±3.7)、(17.8±3.2)比(8.4±1.9)μg/μL]明显增高(均P<0.05);母鼠体质量增加值(孕19d体质量-孕14d体质量)[(29.6±4.9)、(42.7±6.9)比(79.4±8.0)g]、仔鼠存活数[(1.2±0.8)、(3.1±0.8)比(12.0±1.6)只]、仔鼠体质量[(1.8±0.1)、(1.7±0.2)比(2.6±0.1)g]、母鼠存活率(50%、80%比100%)、胎盘总超氧化物歧化酶活力[(98.2±7.0)、(99.2±3.6)比(145.0±3.5)U/mg pro]下降(均P<0.05)。与RUPP经典手术组比较,RUPP改良手术组母鼠体质量增加值、仔鼠存活数、母鼠存活率增高(均P<0.05),而血压、尿蛋白、仔鼠体质量差异无统计学意义(均P>0.05)。结论 RUPP诱发妊娠高血压大鼠模型是研究妊娠高血压的良好模型。RUPP改良手术诱发妊娠高血压效果与RUPP经典手术相似。Objective To investigate the feasibility of creating gestational hypertension models induced by modified surgery for uteroplacental ischemia in pregnant rats （Spragu Dawley）. Methods Thirty pregnant rats were ran domly divided into three groups （10 in each group） ： sham operation group, classic surgery for reducing uterine per fusion pressure （RUPP） group （silver clips were placed in abdominal aortas and bilateral ovarian arteries of rats on the 14th day of their pregnancy） , and modified RUPP group （silver clips were placed in abdominal aorta and unilat eral ovarian artery of rats on the 14th day of their pregnancy）. Results On the 19th day of gestation, compared with those in sham operation group, the systolic blood pressure [（157.4±11.8）, {161.4±6.3） vs （117.0± 9.4）mm Hg-1, diastolic blood pressure [（122.6±8.1）, （123.3±7.7） vs {100.7±4.4）mm Hg], heart rate [{398.4±10.1}, （400.5±13.1） vs （383.9±11.5） beat/min-], urinary protein [（18.1±3.7）, （17.8±3.21 vs （8.4± 1.9）μg/μL, all P〈0.05] in classic and modified RUPP groups were significantly increased, while the mater- nal weight gain [.（29.6±4.9}, （42.7±6.9} vs {79.4±8.0}g＇l, pups survival numbers [{1.2±0.8}, （3.1±0.8} vs （12.0±1.6）-], the weight of pups [,{1.8±0.1}, （1.7±0.2） vs （2.6±0.1）g], the maternal survival rate （50%, 80% vs 100% ）, and the activity of total superoxide dismutase （T-SOD） in placenta [（98.2±7.0）, （99.2±3.6） vs （145.0±3.5）U/rag pro] were declined（all P〈0.05）. Compared with classic RUPP group, the maternal weight gain, pups survival numbers, maternal survival rate were higher in modified RUPP group（all P〈0.05）, but the blood pressure, urinary protein, and the weight of offspring were no significant difference. Conclusions Re duced uterine perfusion pressure rat model is good for studying gestational hypertension. Modified RUPP surgery can successfully induce gestational hypertension, which is similar to the

关 键 词：高血压 妊娠 缺血 动物模型 

分 类 号：R714.246[医药卫生—妇产科学]