The stress response factor RpoS is required for the natural transformation of Escherichia coli  

作　　者：Yan Zhang Mengyue Guo Ping Shen Zhixiong Xie 

机构地区：[1]Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), State Key Laboratory of Virology, College of Life Sciences, Wuhan University

出　　处：《Chinese Science Bulletin》2014年第5期521-527,共7页

基　　金：supported by the National Basic Research Program of China(2013CB933904);the National Natural Science Foundation of China(30971573,21272182);the Science Fund for Creative Research Groups of NSFC(20921062)

摘　　要：The natural transformation of Escherichia coli is a novel and recently developed system that has significance for genetic studies and the biological safety of genetic engineering.However,the mechanisms of transformation,including development of competence and DNA uptake,are not thoroughly understood.In this study,we demonstrated the effect of the general stress response regulator RpoS,which has been associated with E.coli transformation,on natural transformation performed in an‘‘open system’’.We find that RpoS is required for natural transformation but not to artificial transformation and RpoS mainly affect transformation in the liquid culture prior to plating.In the liquid culture,RpoS over-expression promotes natural transformation in early exponential phase and static incubation accumulates RpoS and promotes transformation to a limited extent.These findings provide detailed understanding of RpoS function on natural transformation.The natural transformation of Escherichia coli is a novel and recently developed system that has signifi- cance for genetic studies and the biological safety of genetic engineering. However, the mechanisms of transformation, including development of competence and DNA uptake, are not thoroughly understood. In this study, we demonstrated the effect of the general stress response regulator RpoS, which has been associated with E. coli transformation, on natural transformation performed in an ＂open system＂. We find that RpoS is required for natural transformation but not to artificial transformation and RpoS mainly affect trans- formation in the liquid culture prior to plating. In the liquid culture, RpoS over-expression promotes natural transfor- mation in early exponential phase and static incubation accumulates RpoS and promotes transformation to a limited extent. These findings provide detailed understanding of RpoS function on natural transformation.

关 键 词：大肠杆菌 转化 响应系数 天然 改造自然 应力 大肠埃希氏菌 开放系统 

分 类 号：Q78[生物学—分子生物学]