L-肉碱分子表面印迹聚合物的制备及其识别性能研究  

Preparation of L-Carnitine Molecularly Imprinted Polymers and Recognition Charateristics

作　　者：侯蔺桐[1] 陈娇娇[2] 傅宏俊[3] 薛静[2] 程立伟[2] 

机构地区：[1]天津工业大学材料科学与工程学院,天津300387 [2]天津工业大学环境与化学工程学院中空纤维膜材料与膜过程省部共建国家重点实验室培育基地,天津300387 [3]天津工业大学纺织学部,天津300387

出　　处：《高分子学报》2014年第3期385-392,共8页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号20904039和51003075);天津市应用基础及前沿技术研究计划(项目号10JCYBJC02900)资助项目

摘　　要：以L-肉碱(L-carnitine)作为印迹模板分子,甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,采用硅胶表面分子印迹技术合成了L-肉碱分子印迹聚合物(MIP)和非印迹聚合物(NMIP).通过紫外光谱研究了MAA与L-肉碱之间的结合作用.利用IR和SEM测试分别对产物进行了结构表征和表面形貌观察,说明分子印迹聚合物成功接枝到了硅胶表面.吸附动力学实验结果表明,MIP对L-肉碱有较好的识别性和吸附性;Scatchard分析表明该印迹聚合物中存在着一类等价的结合位点,最大表观结合量为71.00μmol/g,离解常数Kd=2.76×10-4mol/L;选择性吸附实验结果表明,MIP对L-肉碱的吸附结合量高于其D型异构体和其他类似物;拆分实验结果表明,MIP对DL-肉碱的拆分有一定作用.A molecularly imprinted polymer （MIP） with specific molecule identification properties for L-carnitine was synthesized on the surface of modified silica, using L-carnitine as template molecule, ethyleneglycol dimethacrylant （EGDMA） as the cross-linker, methacrylic acid （MAA） as functional monomer and 2,2＇-azobisisobutyronitrile （AIBN） as the initiator respectively. The binding effect of the complex formed between the template and the functional monomer were obtained by UV spectrophotometry. The MIP was characterized by IR spectroscopy and SEM, and the results demonstrated the formation of a grafted polymer layer on the modified silica gel surface.The absorption kinetics experiments indicated that compared with non-imprinted polymer, MIP showed better recognition ability and absorptivity. Moreover, the polymer was investigated in static adsorption equilibrium experiments and the Scatchard analysis to evaluate the adsorption characteristics. The results showed that the homogeneous binding sites were formed in the MIP, and the dissociation constants （Kd） and the apparent maximum number （Qmax） of binding sites were estimated to be 2.76×10-4 mol/L and 71.00 μmol/g, respectively.The selective binding experiment for substrates shows that the affinity and selectivity of the MIP for L-carnitine are higher than those for D-carnitine and two other different substrates. MIP also showed good selectivity for L-carnitine through the resolution experiments.

关 键 词：分子表面印迹 L-肉碱 甲基丙烯酸 手性识别 特异吸附 

分 类 号：O631.3[理学—高分子化学]