EGCG对酒精性肝病小鼠肝脏铁调素表达影响  

Effects of epigallocatechin-3-gallate on expression of hepcidin mRNA in liver of mice with alcoholic liver disease

作　　者：韦远欢 施文祥[1] 任源[1] 

机构地区：[1]桂林医学院公共卫生学院,广西桂林541004

出　　处：《中国公共卫生》2014年第3期305-307,共3页Chinese Journal of Public Health

基　　金：广西自治区教育厅项目(200103YB090);桂林医学院"项目驱动教学法"专项课题

摘　　要：目的探讨表没食子儿茶素没食子酸酯（EGCG）对酒精性肝病（ALD）小鼠肝脏铁调素（hepcidin）mRNA表达影响。方法6—8周龄雄性SPF级C57／BL6小鼠随机分为对照组和造模组，造模组小鼠每日给予乙醇灌胃，并于造模第9周随机分为模型组，EGCG10、20、30mg/kg组，4周后处死小鼠，观察各组小鼠肝脏病理变化，测定谷丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平、肝脏铁含量，采用real—timePCR方法检测肝组织铁调素mRNA表达。结果与对照组比较，模型组小鼠血清ALT、AST[分别为（237．25±50．26）、（442．38±56．31）u／L]水平升高（P〈0．01）；肝脏病理观察显示，肝细胞呈中度脂肪变性；肝组织铁含量显著升高，肝脏铁调素mRNA表达[0．008±0．002]明显降低（P〈0．01）；与模型组比较，EGCG组小鼠血清ALT、AST水平[分别为（53．75±6．67）、（151．75±13．81）U／L]明显下降（P〈0．01）；肝铁含量及肝脏hepcidinmRNA[0．051±0．011]表达显著升高（P〈0．01）。结论结论EGCG可以上调ALD小鼠肝脏铁调素mRNA表达，抑制小肠铁吸收，对酒精性肝病具有一定保护作用。Objective To study effects of epigallocatechin-3-gallate （EGCG） on hepcidin mRNA in liver of mice with alcoholic liver disease （ALD） and to explore its mechanism. Methods C57/BL6 mice were randomly divided into a normal group and a model group. Alcoholic liver disease was induced by gavage of alcohol for 12 weeks. At the end of 8 weeks, the alcohol group was divided into a model group and three EGCG groups （ 10,20, and 30 mg · kg^ -1 ）. The mice in the EGCG groups received an intraperitoneal injection of EGCG and simultaneous intragastric administration of alcohol for 4 weeks. Liver injuries were assessed with histopathologic examination and serum alanine aminotransferase （ALT）, aspartate aminotransferase （AST） levels. In addition, liver iron levels were evaluated. Hepcidin mRNA in liver tissue was also determined with real-time PCR. Results The mice in model group had marked increases in serum ALT, AST levels and liver iron concentration compared with normal group, and their liver tissues showed moderate hepatocyte fatty degeneration. But the mice in EGCG groups had decreased ALT, AST levels and liver iron concentration and im- proved pathological changes. Liver hepcidin mRNA expression level was decreased significantly in model group compared with the normal group,but markedly increased in EGCG treatment groups. Conclusion Compared with model group,the hepcidin mRNA expression in livers of EGCG treatment ALD mice increases, and EGCG may play a protective role in the development of ALD. The possible mechanism of the effect may relate to EGCG inhibiting iron absorption in small intestine by the upregulation of hepcidin.

关 键 词：没食子儿茶素没食子酸酯(EGCG) 酒精性肝病(ALD) 铁过载 铁调素(hepcidin) 

分 类 号：R114[医药卫生—卫生毒理学]