阿维菌素与高效氯氰菊酯联合对大鼠脂质过氧化影响  

Combined effect of avermectine and beta-cypermethrin on lipid peroxidation in rats

作　　者：冯小雨[1] 安连杰 焦冬[1] 周晓蓉[1] 

机构地区：[1]哈尔滨医科大学公共卫生学院,黑龙江哈尔滨150081

出　　处：《中国公共卫生》2014年第3期308-311,共4页Chinese Journal of Public Health

摘　　要：目的通过动物实验观察阿维菌素与高效氯氰菊酯的联合毒性作用,并从氧化损伤的角度探讨其联合作用的机制。方法将大鼠随机分为4组,分别为对照组、阿维菌素组、高效氯氰菊酯组以及混合组,灌胃染毒30 d,观察大鼠一般状况及体重变化,计算脏器系数及检测血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量。结果混合组大鼠中毒症状较各单剂组加重,持续时间延长;与对照组比较,阿维菌素组、高效氯氰菊酯组及其混合组体重均降低,仅雄性大鼠体重差异有统计学意义(P<0.05);阿维菌素组、高效氯氰菊酯组及其混合组大鼠血清中MDA含量显著增加,SOD和GSH-Px酶活力显著降低(P<0.05);析因分析结果显示,阿维菌素和高效氯氰菊酯对大鼠血清中MDA含量(F=0.324,P=0.57),SOD(F=2.86,P=0.09)和GSH-Px(F=2.29,P=0.14)酶活力没有交互作用,联合作用方式均为相加作用。结论阿维菌素与高效氯氰菊酯联合作用毒性较各单剂量组增加,氧化损伤可能是其联合毒性作用机制之一。Objective To observe combined toxicological effect of avermectine and beta-cypermethrin in rats and to explore the mechanism in oxidative damage of the effect. Methods Forty Wistar rats were randomly divided into 4 groups （normal control, avermectine, beta-cypermethring, and avermectine plus beta-cypermethrin group ）. The rats were treated by gavage for 30 days and their general condition and changes in body weight were observed. By the end of the experiment, the organ coefficients of the rats were calculated and the activities of glutathione peroxidase （GSH-Px） and superoxide dismutase（ SOD ）, and the contents of malondialdehyde（MDA） in serum of the rats were detected. Results Compared with those of single-dose groups, the poisoning symptoms in the avermectine plus beta-cypermethrin group were more severe and prolonged. Compared with the control group, the rats in the three exposure groups had decreased body weight, but only the body weight reduction in male rats was statistically significant（ P 〈 0. 05 ）. Compared with the control group,the contents of serum MDA significantly increased and the activities of SOD and GSH-PX dramatically reduced（ P 〈 0. 05 ） in the three exposure groups. Factorial analysis showed that the combined mode of the effects was additive between avermectine and beta-cypermethrin for the contents of serum MDA（ F = 0. 324 ,P = 0. 57 ） and the activ- ities of SOD （F=2. 86,P =0. 09） and GSH-Px（F =2.29,P =0. 14）. Conclusion Combined effect of avermectine and beta-cypermethrin is more toxic than that of single dose exposure and oxidative damage may be one of the mechanisms of the combined toxicological effect.

关 键 词：阿维菌素 高效氯氰菊酯 脂质过氧化 

分 类 号：R994.39[医药卫生—毒理学]