GC法测定痛宁凝胶中冰片和薄荷脑的含量  

Determination of borneol and menthol in Tongning gel by GC

作　　者：刘莉莉[1] 秦建平[1] 杨素德[1] 韦迎春[1] 李雪峰[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室,连云港222001

出　　处：《中国药科大学学报》2014年第1期76-78,共3页Journal of China Pharmaceutical University

基　　金：国家"重大新药创制"科技重大专项资助项目(No.2013ZX09402-203)~~

摘　　要：采用GC法同时测定痛宁凝胶中龙脑、异龙脑和薄荷脑的含量。采用5%二苯基-95%二甲基硅烷共聚物为固定相的毛细管色谱柱（30 m×0.32 mm×0.2 μm），分流比为50∶1，柱温为88 ℃。结果表明痛宁凝胶中的龙脑、异龙脑和薄荷脑的分离度和线性关系良好，线性范围依次为0.116 9~1.402 8 mg/mL、0.107 8~1.293 6 mg/mL和0.112 7~1.352 4 mg/mL；加样回收率依为98.02%（RSD=1.76%）、100.00%（RSD=1.78%）和101.77%（RSD=1.33%）。所建立的方法适合于痛宁凝胶的质量控制。To establish a GC method for the determination of borneol, isobornel and mentol in Tongning gel. The GC column was packed with 5% diphenyl and 95% dimenthyl polysiloxanc（30 m×0. 32 mm×0. 2 μm）, and the split ratio and column temperature were 50 ∶1 and 88°C, respectively. The results showed that borneol, isoborneol and menthol in Tongning gel were well-separated. The calibration curves were linear over the concentration ranger of 0. 116 9-1. 402 8mg/mL, 0. 107 8-1. 293 6mg/mL and 0. 112 7-1. 352 4 mg/mL for borneol, isoborneol and menthol, respectively. The average recoveries were 98. 02%（RSD=1. 76%）, 100. 00%（RSD=1. 78%）and 101. 77%（RSD=1. 33%）respectively. The established method is accurate, sensitive and reproducible in the quality control of Tongning gel.

关 键 词：GC法 薄荷脑 龙脑 异龙脑 痛宁凝胶 含量测定 

分 类 号：R286.0[医药卫生—中药学]