宁玉草莓茎尖组培快繁技术研究  

Study on Stem-tip Tissue Culture and Rapid Propagation Techniques of Strawberry “Ningyu”

作　　者：夏瑾[1] 赵密珍[1] 孟宪凤[1] 

机构地区：[1]江苏省农业科学院园艺研究所,江苏南京210014

出　　处：《江西农业学报》2014年第3期16-17,21,共3页Acta Agriculturae Jiangxi

基　　金：农业科技成果转化资金项目(2012GB2C100159)

摘　　要：以宁玉草莓匍匐茎为外植体，进行消毒灭菌、茎尖分化、继代增殖和生根培养基筛选研究，结果表明：最佳消毒方法为75％乙醇40 s＋0．1％升汞6～8 min；草莓茎尖分化培养基：MS＋6-BA 0．5 mg/L＋NAA 0．1 mg/L；继代增殖培养基：MS＋6-BA 0．5 mg/L＋NAA 0．1 mg/L，其增殖系数为9；生根培养基：1/2MS＋NAA 0．05 mg/L，生根率可达96．6％。The stolons of strawberry variety “Ningyu” were used as explants .After disinfection , we studied and selected the appropriate culture media for stem -tip differentiation, subcultural propagation and rooting .The results showed that the best method of disinfection was using 75%ethanol for 40 s plus 0.1%HgCl2 for 6~8 min;the best differentiation medium was MS ＋0.5 mg/L 6-BA＋0.1 mg/L NAA;the best medium for subcultural propagation was MS ＋0.5 mg/L 6-BA＋0.1 mg/L NAA, and its multiplication coefficient was 9;the best rooting medium was 1/2MS＋0.05 mg/L NAA, and the rooting rate reached 96.6%.

关 键 词：宁玉草莓 组织培养 快繁技术 

分 类 号：S668.4[农业科学—果树学]