大鼠心脏移植后动态监测血清RANTES和单核细胞趋化蛋白-1的意义  

The changes of serum RANTES and MCP-1 following cardiac transplantation in rats and implication

作　　者：顾晓[1] 唐孝达[1] 杨尚琪[1] 刘永[1] 周佩军[1] 徐达[1] 王祥慧[1] 谭建明[1] 

机构地区：[1]上海市第一人民医院泌尿外科,200080

出　　处：《中华器官移植杂志》2001年第1期36-38,共3页Chinese Journal of Organ Transplantation

基　　金：上海市医学领先专业重点学科发展基金资助项目

摘　　要：目的　探讨测定血清RANTES(regulatedonactivationnormalTcellexpressedandsecreted)和单核细胞趋化蛋白 1(MCP 1)浓度在判断大鼠心脏移植急性排斥反应中的意义及环孢素A(CsA)对它们的影响。方法　施行大鼠异位心脏移植术 ,按术后免疫抑制方案的不同将动物分为未干预组 (不给免疫抑制剂 )、低剂量CsA组、高剂量CsA组 ,并设同系移植对照组 ;采用酶联免疫吸附法检测受者术前及术后不同时段血清RANTES和MCP 1的浓度。结果　血清RANTES浓度的动态变化与急性排斥反应的进程相关 ,峰值出现于排斥反应的早期 ;应用CsA者 ,其RANTES峰值出现延缓 ,且明显低于未干预组 (P <0 .0 5 ) ;血清MCP 1的浓度在术后 6h及严重急性排斥反应时 2次升高 ,应用CsA后第一峰出现延迟 ,但不影响其峰值浓度 (P >0 .0 5 ) ,高CsA组未出现第二个高峰。结论　术后动态观察受者血清RANTES和MCP 1的浓度 。Objectives To investigate the implication of serum regulated on activation normal T cell expressed and secrected (RANTES) and MCP 1 in cardiac acute allograft rejection and the effect of Cyclosporin A (CsA). Methods Four groups of rats underwent the heterotopic cardiac transplantation: untreated group, low dose of CsA group, high dose of CsA group and isograft group. The serum RANTES and MCP 1 of recipients were detected by using ELISA method. Results The changes in serum RANTES were correlated with the process of acute rejection. RANTES concentrations peaked in the early stage of acute graft rejection and CsA could delay the peak. The serum peak level of RANTES was significantly lower than that in untreated group ( P < 0.05 ). The serum MCP 1 concentrations peaked 6 h after operation and in severe stages of acute rejection respectively. The first peak of MCP 1 reached later by using CsA, but CsA did not affect its peak level ( P > 0.05 ). The second peak of MCP 1 was decreased or did not occurred in the CsA treated groups. Conclusions RANTES concentrations were increased in the early episodes of acute rejection and could be used as a marker for diagnosis of acute rejection. MCP 1 not only involved in the ischemia/reperfusion injury but also severe acute rejection. It may be the important mechanisms of CsA to intervene the acute rejection by decreasing the release of RANTES and inhibiting its immunological effects.

关 键 词：移植物排斥 心脏移植 单核细胞趋化蛋白-1 RANTES 

分 类 号：R654.2[医药卫生—外科学]