A Genetic Transformation System for Rosa multiflora Thunb. var. cathayensis Rehd. et Wils through Callus Induction  

红刺玫愈伤组织诱导的遗传转化体系建立(英文)

作　　者：张冬梅[1] 姜灵敏[1] 罗玉兰[1] 覃仁娟 

机构地区：[1]上海市园林科学研究所,上海200232 [2]四川农业大学,四川成都611830

出　　处：《Agricultural Science & Technology》2014年第1期1-5,共5页农业科学与技术（英文版）

基　　金：Supported by the State Bureau of Forestry 948 Project(P2009-4-25)~~

摘　　要：An efficient genetic transformation system is a preparation for Rosa multi-flora Thunb. var. cathayensis Rehd. et Wils to diversify its flower color through ge-netic engineering. We firstly optimized the explants and culture conditions on callus induction, hormone concentrations and dark period of culture time on bud differentia-tions in particular, with sterilized seedlings to establish the regeneration system of R. multiflora. It showed that callus induction frequency reached 100% after the ex-plants being cultured in dark for 21 d when MS was chosen to be the initial culture medium. The bud differentiation rate was 48% after cal i being cultured under dark for 8 d on MS medium supplemented with TDZ （1.5 mg/L） and NAA （0.05 mg/L）. The cal i was used as the explants that were infected with Agrobacterium tumefa-ciens harboring a DFR-RNAi construct. The transformation rate reached as high as 50%. The establishment of a highly efficient rose gene transformation system out-lined in this report is prerequisite for genetic improvement in rose flower colors.以红刺玫无菌苗为初始材料,通过探讨外植体、培养条件,以及激素配比浓度和暗培养时间对愈伤组织分化芽的影响,建立了红刺玫叶片愈伤组织诱导的再生体系:MS为初始培养基,暗培养21 d,愈伤组织诱导率达到100%。分化培养基为MS+TDZ 1.5 mg/L+NAA0.05 mg/L,暗培养8 d,芽分化率达48%;通过遗传转化条件优化,建立了以红刺玫愈伤组织为转化受体,通过根癌农杆菌介导,以红刺玫的DFR-RNAi为表达载体,GUS为标记基因的遗传转化体系,转化效率达到50%。

关 键 词：Rosa multiflora Callus induction Callus proliferation Genelic transfor-mation system 

分 类 号：S685.12[农业科学—观赏园艺]