1-(2,6-二甲基苯氧基)-2-(3,4-二甲氧基苯乙氨基)丙烷盐酸盐对肺血管周细胞增殖与免疫表型的影响  

Effects of DDPH on proliferation and immunophenotypes of the pulmonary vascular pericytes

作　　者：袁永辉[1] 车东媛[1] 熊密[1] 

机构地区：[1]同济医科大学基础医学院病理学教研室,武汉430030

出　　处：《中华结核和呼吸杂志》2001年第2期101-104,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　观察具有抗高血压作用新药 1 (2 ,6 二甲基苯氧基 ) 2 (3,4 二甲氧基苯乙氨基 )丙烷盐酸盐 (DDPH)对低氧内皮细胞条件培养液 (HECCM)诱导的大鼠肺血管周细胞 (PC)增殖和免疫表型转化有无抑制作用 ,为开发具有降压和防止肺血管构型重建新药物提供实验依据。方法　实验根据不同条件培养液分为 4组 :常氧内皮细胞条件培养液 (NECCM)组 ,NECCM +DDPH组 ,HECCM组和HECCM +DDPH组。应用细胞培养、免疫细胞化学染色、图像分析及流式细胞术观察HECCM和DDPH对PC平滑肌肌动蛋白 (α SM actin)和增殖细胞核抗原 (PCNA)的表达及细胞周期的影响。结果　①HECCM组PCα SM actin呈强阳性表达 ,CD34 和S 10 0呈阴性反应 ,而其它各组PCα SM actin、CD34及S 10 0均呈阳性表达 ;②HECCM组PCα SM actin和PCNA的表达量分别是NECCM组的 1 32倍 (F=11 0 9,P =0 0 0 0 1)和 1 2 4倍 (F =2 89,P =0 0 2 5 7) ,是HECCM +DDPH组的 1 30倍 (F =3 6 5 ,P =0 0 0 70 )和 1 2 1倍 (F =2 6 3,P =0 0 414) ;③HECCM组PC的G0 G1期细胞百分率分别比NECCM组和HECCM +DDPH组低 11 7%和 9 1% ,S期细胞百分率分别高 5 6 %和 4 2 % ,G2 M期细胞百分率分别高 6 1%和 4 9% ;④DDPH对HECCM引起的PCs合成α SM actin和PCNA增高?Objective To study the effects of 1 (2,6 dimethylphenoxy) 2 (3,4 dimethoxyphenylethylamino) propane hydrochloride (DDPH) on proliferation and immunophenotypes of newborn rat pulmonary vascular pericytes (PCs) induced by hypoxic endothelial cell conditioned medium (HECCM) from porcine pulmonary arteries. Methods The PCs were divided into 4 groups according to the endothelial cell conditioned medium (ECCM):normoxic ECCM (NECCM) group,NECCM+DDPH group,HECCM group and HECCM+DDPH group Cell culture,immunocytochemical staining,image analysis and flow cytometry were used to investigate the effects of HECCM and DDPH on the expression of α smooth muscle actin (α SM actin) and proliferating cell nuclear antigen (PCNA) and cell cycle in PCs. Results The α SM actin antigen in the PCs in HECCM group was more positively expressed than that in the other three groups,but CD 34 antigen and S 100 antigen were negatively expressed The expression of α SM actin antigen,CD 34 and S 100 was positive in the groups of NECCM,NECCM+DDPH and HECCM+DDPH.The expression of α SM actin and PCNA in HECCM group was 1 32 times ( F =11 09, P =0 000 1) and 1 24 times ( F =2 89, P =0 025 7) that in NECCM group,1 30 times ( F =3 65, P =0 007 0) and 1 21 times ( F =2 63, P =0 041 4) that in HECCM+DDPH group,respectively;The percentage of the cells in the G 0 G 1 phase in the HECCM group was lower by 11 7% and 9 1%,in S phase higher by 5 6% and 4 2%,in G 2 M phase higher by 6 1% and 4 9% than that in the groups of NECCM,HECCM+DDPH,respectively. The inhibitory rates of DDPH on the increased α SM actin and PCNA syntheses in PCs induced by HECCM were 23 4% and 17 1% respectively The inhibitory rate on the increased PCs from G 0 G 1 phase to S phase was 8 3%. Conclusion DDPH might directly inhibit PCs from proliferation and immunophenotypical transformation of smooth muscle like cells induced by HECCM

关 键 词：丙烷盐酸盐 周细胞 高血压 降压作用 肺动脉高压 

分 类 号：R965[医药卫生—药理学]