PLC法测定兔眼房水中包甲素类似物-ATST的含量  

A HPLC Method for Determination of the Content of an analog of Baogongteng A-ATST in the Aqueous Humor of Rabbits

作　　者：杨丽敏[1] 谢一凡[1] 王惠宁[1] 

机构地区：[1]上海第二医科大学化学教研室,上海200025

出　　处：《上海第二医科大学学报》2001年第1期20-21,共2页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市教委重点学科发展基金资助(S99209)

摘　　要：目的建立兔眼房水中包甲素类似物ATST含量的高效液相色谱 (HPLC)测定法。　方法以奥美拉唑(OMZ)为内标 ,流动相组成为甲醇 - 1%三乙胺 (5 7∶43) ,紫外检测波长为 2 35nm。　结果兔眼房水样品中药物浓度在2 .0 5 6～ 41.12 μg/ml范围内线性关系良好 ,r =0 .9997,平均回收率为 94.5 8% ,日内和日间标准偏差 (RSD)分别小于5 %和 10 % (n =5 )。　结论本方法稳定、可靠 ,可用于ATST的药动学研究。Objective A HPLC method was established for determination of the content of ATST in the aqueous humor of rabbits. Methods The mobile phase was consisting of methanol-1% triethylamine(57∶43) and omeprazole (OMZ) as internal standard.The detection was carried out with an ultraviolet detector operated at 235nm. Results There was linearity over the range of 2.056 ～ 41.12 μg/ml in the humor aquosus,r=0.9997.The average recovery of ATST was 94.58%.Intra-day and inter-day RSD were less than 5% and 10%(n=5),respectively. Conclusion The method is reliable.It can be used for the study on the pharmacokinetics of ATST.

关 键 词：包甲素类似物 高效液相色谱 6-乙酰氧基-3-苯磺酰氧基莨菪烷 

分 类 号：R96[医药卫生—药理学]