骨形成蛋白2基因转染的人牙周膜成纤维细胞的生物学特性  

The Biological Characterization of Bone Morphogenetic Protein 2 Gene Transfected Human Periodontal Ligament Fibroblasts

作　　者：司晓辉[1] 刘正[1] 

机构地区：[1]上海第二医科大学第九人民医院口腔内科,上海200011

出　　处：《上海第二医科大学学报》2001年第1期24-27,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助项目(30000191);中国博士后科学基金资助项目(中国博基[1999]17);上海市博士后科研资助计划[沪科(2000)第478号]

摘　　要：目的建立表达骨形成蛋白 2 (BMP2 )的人牙周膜成纤维细胞 (HPDLFs)并观察其生物学特性。方法利用脂质体将BMP2噬菌粒表达载体pBK -B2转染至HPDLFs,免疫组化ABC法检测BMP2基因的表达 ,并检测转染细胞的碱性磷酸酶 (ALP)活力、骨钙素 (OC)含量和矿化能力。　结果转染BMP2基因后 ,HPDLFs内有BMP2蛋白的表达 ,ALP活力、OC含量和矿化结节数量均显著增加。　结论BMP2基因在HPDLFs中得到表达并促进其向成骨样细胞分化。Objective To establish the human periodontal ligament fibroblasts(HPDLFs)that express BMP2 and observe their biologicl characterization. Methods A phagemid expression vector for BMP2 (pBK-B2) was transfected into HPDLFs by using LipofectAMINE. The BMP2 expression was determined by the immunohistochemical ABC method. The alkaline phosphatase (ALP) activity, osteocalcin (OC) production and capacity of mineralization were measured in the transfected cells. Results BMP2 protein was expressed in HPDLFs after gene transfection. The BMP2 gene transfected cells showed prominently elevated ALP activity,OC production and increase in mineralized nodules. Conclusion The results indicate that BMP2 is expressed in HPDLFs and is involved in inducing differentiation of HPDLFs into osteoblast-like cells.

关 键 词：骨形成蛋白 人牙周膜成纤维细胞 基因转染 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]