姬松茸多糖对小鼠骨髓来源树突状细胞抗肿瘤作用的影响  

Effect of Agaricus Blazei Polysaccharide on Anti-tumor Effect of Mouse Bone Marrow-derived Dendritic Cells

作　　者：秦晓飞[1] 赵明耀[2] 董子明[2] 

机构地区：[1]河南省洛阳正骨医院,河南洛阳471002 [2]郑州大学基础医学院,河南郑州450052

出　　处：《中医药导报》2014年第2期74-76,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:研究姬松茸多糖对小鼠骨髓来源树突状细胞抗肿瘤功能的影响,为临床应用提供理论依据。方法:体外培养小鼠骨髓来源DC,加入ABP溶液,流式细胞仪检测DC表面CD86和CD11a的表达,混合淋巴细胞反应检测ABP对DC抗原提呈能力的影响,MTT法检测ABP对细胞毒性T淋巴细胞(CTL)对H22肝癌细胞杀伤功能的影响。结果:中剂量ABP组(100μg/mL)DC表面CD86、CD11a表达上调;CTL对肿瘤细胞的杀伤率升高,差异有统计学意义(P<0.05)。结论:适当浓度的ABP能促进DC分化成熟,增强DC的抗肿瘤作用。Objective： To observe the regulation on the functional of dendritic cells （DC） derived from murine bone-marrow by Agaricus blazei polysaccharides （ABP） in vitro and provide the experiment basis for clinical application. Methods： Different dosages of ABP were added to the culture medium, the expression of CD86 and CDlla were analyzed with a flow cytometer; Killing capa- bilities of allogeneic T cells induced by DC were examined with MTT method. Killing capability of cytotoxicity T lymphocyte on H22 hepatocellular carcinoma ceils was examined with MTT. Results： The expression of CD86 and CDlla as well as killing capability of eytotoxicity T lymphocyte increased in the middle dose of ABP group, with significant difference （P〈0.05）. Conclusions： The suitable concentration of ABP can promote the maturation of DC and enhance the antitumor effect of DC.

关 键 词：姬松茸多糖 树突状细胞 抗肿瘤 

分 类 号：R285.5[医药卫生—中药学]