三种冷冻保护剂对人精子活力及生育潜力影响的研究  

Experiment study on the effect of three cryopreservative mediums on the motility and the latent fertilizing ability of human spermatozoa

作　　者：张巧玉[1] 史常旭[1] 罗萍[1] 蔡敏[1] 

机构地区：[1]第三军医大学西南医院妇产科,重庆400038

出　　处：《重庆医学》2001年第1期25-26,28,共3页Chongqing medicine

摘　　要：目的　通过比较甘油、甘油—卵黄—柠檬酸钠 (GYG)和甘油—卵黄—柠檬酸钠—L—谷氨酰胺 (GYCG)三种冷冻保护剂 (CPM)的冷冻保护效果 ,旨在寻求简单、安全、有效、经济的精子冻贮方法。方法　应用甘油、GYG和GYCG三种冷冻保护剂(CPM) ,通过冻贮前后精液常规分析 ,精尾低渗肿胀率 (HOSR)、精子穿卵率 (SPR)等精子功能的检测指标评价三种CPM的冷冻保护效果。结果　三种CPM对精子功能的冷冻保护作用各不相同 ,但GYCG较其他两种CPM具有更显著的保护人精子功能的作用。结论　在三种CPM中 ,GYCG的冷冻保护效果明显优于GYC和单一甘油 ,预示应用GYCG作为CPM可使人精子保持较高的生育潜力 ,具有安全、价廉、简单等优点 。Ojuective To charify the different effect of different cryoprotective mediums(CPMs) on human spermatozoa.Methods Conventional semen analysis, spermatozoa hypo osmotic swelling rate(HOSR), spermatozoa in vitro penetrate zona free hamster ova rate(SPR) were used to evaluate the cryoprotective effect of different CPMs, glycerol,glycorol eygyolk sodium(GYC) and GYC glutamine(GYCY)on human spermatozoa.Results During the procedure of cryoprotective and thawing different CPM has different protective effect on the spermatozoa. Among them, GYCG is superior to the other two CPMs as far as maintaning the functional intergrity of sperm.Conclusion GYCG has better and effective cryoprotective effect than glycerol and GYC, and we suggest it be used in clinical practice.

关 键 词：冷冻保护剂 人精子 精子活力 生育潜力 甘油-卵黄-柠檬酸钠 

分 类 号：R321-33[医药卫生—人体解剖和组织胚胎学]