积雪草甙对体外培养的成纤维细胞的作用  

The Effects of Asiaticoside on Fibroblasts in vitro Culture

作　　者：谢举临[1] 利天增[1] 祁少海[1] 黎志明[1] 梁惠珍[2] 吴义方[3] 

机构地区：[1]中山医科大学附属第一医院烧伤科,广东广州510080 [2]中山医科大学病理学教研室 [3]中山医科大学电子显微镜室,广东广州510089

出　　处：《中山医科大学学报》2001年第1期41-43,47,共4页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：广东省重点攻关项目资助! (982 78172 )

摘　　要：【目的】探讨积雪草甙治疗增生性瘢痕的作用机制。【方法】采用光镜、电镜、3 H TdR、3 H脯氨酸掺入及MTT比色等方法检测成纤维细胞核DNA合成和胶原蛋白的合成。【结果】积雪草甙不仅能影响成纤维细胞的超微结构 ,而且能抑制成纤维细胞增殖及胶原蛋白的合成。【结论】积雪草在预防和治疗增生性瘢痕中有重要作用 ,其机制可能与抑制成纤维细胞增殖和胶原蛋白合成有关。Objective To investigate the possible mechanism of the asiaticoside used for the treatment of hypertrophic scar. Method Using microscope, electron microscope, MTT test, 3H-TdR and 3H-Proline incorporation to study the effect of the asiaticoside on DNA synthesis and collagen biosynthesis of fibroblast in vitro culture. Results The asiaticoside not only affected the ultrostructure of fibroblast, but also inhibted the DNA synthesis and collagen biosynthesis. Conclusion Asiaticoside can play an important role in the prevntion and treatment of scars, the mechanism may be that the asiaticoside can inhibted the DNA synthesis and collagen biosynthesis of fibroblast.

关 键 词：积雪草甙 增生性瘢痕 药物疗法 治疗 成纤维细胞 体外培养 

分 类 号：R619.6[医药卫生—外科学] R285.5[医药卫生—临床医学]