人毛发多肽生殖激素研究  

A preliminary study of polypetide reproductive hormones in human hair.

作　　者：兰君[1] 杨惠钟 孟衍建[1] 万学俊 孔雅玲[3] 

机构地区：[1]四川省计划生育科研所,四川成都610041 [2]四川省环境保护科学研究院,四川成都610041 [3]华西医科大学附属第二医院,四川成都610041

出　　处：《四川医学》2001年第1期26-27,共2页Sichuan Medical Journal

基　　金：四川省科委资助;四川省计生委资助课题

摘　　要：目的　对人毛发是否存在多肽生殖素进行研究 ,并探讨建立用人毛发代替人血清测定多肽生殖激素的方法。方法　用 p H- 7.2～ 7.4的磷酸缓冲液从人毛发中抽提出多肽生殖激素 ,并用常规的放射免疫方法测定。结果　人毛发中存在有黄体生成素 (L H) ,泌乳素 (PRL ) ;洗发剂洗发对人毛发中多肽生殖激素水平无影响 ;人毛发部位 (根、中、梢段 )对人毛发多肽激素水平无影响 ;自身对照的人毛发与人血清中同种多肽激素呈高度显著性相关 ;r(L H) =0 .5 87(n=2 9) P<0 .0 0 1;r(PRL ) =0 .46 7(n=33) ,P<0 .0 0 5。结论　人毛发中存在有多肽生殖激素 (L H,PRL )用人毛发代替人血清测定多肽生殖激素是可行的。Objective The authors investigated whether polypeptide hormones are measurable in human hair and tried to build a method of using human hair to replace serum for determination of polypeptide hormones.Methods Polypeptide hormones were extracted by pH 7.2～7.4 phosphate buffer solution and assayed by the current RIA of DPC instruction.Results [WT5”BZ]In this study,the actual presence of polypeptide hormones(LH,PRL) in human hair was evidenced;the polypeptide hormones value of hair after washing with shampoos were not affected;the polypeptide hormones value of different segments of the hair (top,middle and basal) did not significantly vary;There were highly significant correlations between hair polypeptide hormones and serum polypeptide hormones of healthy human adults:r(LH)=0.587(n=29);r(PRL)=0.467(n=33),P<0.005.Conclusion Polypeptide hormones(LH,PRL)exist in human hair.It is feasible to use human hair as an alternative specimen for determination of polypeptide hormones.

关 键 词：人毛发 多肽激素 放射免疫测定 血清测定 

分 类 号：R341[医药卫生—基础医学]