激光照射对脊髓损伤大鼠TNF-α、IL-6和IL-10表达的影响  

Effect of Low-Level Laser Irradiation on Expression of TNF-α, IL-6 and IL-10 after Acute Spinal Cord Injury in Rats

作　　者：王继猛[1] 梁卓文[1] 胡学昱[1] 焦海斌[1] 王晶[2] 王哲[1] 张镇西[2] 

机构地区：[1]第四军医大学西京骨科医院,陕西西安710032 [2]西安交通大学生命科学与技术学院,陕西西安710032

出　　处：《中国激光》2014年第2期245-249,共5页Chinese Journal of Lasers

基　　金：国家自然科学基金(81070996;11274249);陕西省社发攻关基金(2011K14-07-14)

摘　　要：应用Allen造模法制备大鼠急性脊髓损伤(SCI)模型,研究弱激光照射对损伤脊髓组织内肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)和白介素-10(IL-10)表达的影响。68只SD大鼠随机分为正常组、SCI组和照射组,照射组应用810nm弱激光经皮照射相应的脊髓损伤节段。分别于术后1d、3d和7d进行BBB评分;1h、3h、6h、12h、1d、3d、5d和7d取材,酶联免疫吸附检测(ELISA)法检测受损脊髓组织内TNF-α、IL-6和IL-10的表达情况。实验发现,照射后7d照射组大鼠BBB评分高于SCI组,且有统计学意义(P<0.05);与SCI组相比,照射组受损脊髓组织内TNF-α和IL-6的表达量均降低,且在术后6h、12h、1d(TNF-α)和6h、12h、5d(IL-6)差异有统计学意义(P<0.05);IL-10的表达量在各个时间点均增高,且在术后1d、3d、5d和7d差异有统计学意义(P<0.05)。结果表明弱激光照射能明显抑制脊髓损伤早期前炎症因子TNF-α和IL-6的表达,促进抑炎因子IL-10的表达,促进损伤后期大鼠运动功能的恢复。Abstract Spinal cord injury （SCI） model is created by Allen＇s method, and this study is designed to explore the effect of 810 nm low-level laser irradiation on expressions of TNF-α, IL-6 and IL-10 in acute spinal cord injury. 68 SD rats are randomly divided into normal group, SCI group and irradiation group. Light （810 nm, 150 mW） is applied transcutaneously at the lesion site of rats in irradiation group. Functional recovery is assessed by open-field test （BBB test） on 1 d, 3 d, 7 d after injury. The expressions of TNF-a, IL-6 and IL-10 in the injured spinal cords are examined by enzyme linked immuno sorbent assay （ELISA） method at the time of 1 h, 3 h, 6 h, 12 h, 1 d, 3 d, 5 d and 7 d after injury. We find that there is a statistically significant functional recovery （P〈：0.05） in the irradiation group compared to SCI group at 7 d. The expressions of TNF-α and IL-6 of irradiation group is significantly lower than the comparable SCI group at the time of 6 h, 12 h, 1 d （TNF-a） and 6 h, 12 h, 5 d （IL-6） （P^0.05）. The expression of IL-10 of Laser group is significantly higher than the comparable SCI group at the time of 1 d, 3 d, 5 d and 7 d （P〈0.05）. There is no statistical difference at other time points. The results of our study show that laserirradiation can effectively inhibit the expression of pro-inflammatory cytokine TNF-α and IL-6 and significantly promote the expression of IL-10 in rats of acute spinal cord injuries, and laser irradiation can promote functional recovery of acute spinal cord injury rats.

关 键 词：医用光学 弱激光照射 脊髓损伤 肿瘤坏死因子α 白介素-6 白介素-10 

分 类 号：R318.51[医药卫生—生物医学工程]