氯嘧磺隆分子印迹聚合物的合成及其性能  

Synthesis of chlorimuron-ethyl imprinted polymer and its molecule recognition capability

作　　者：陈萍[1] 孟红莲[1] 蔡青红[1] 郭欣[1] 陈冠华[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《河北大学学报（自然科学版）》2014年第1期40-46,52,共8页Journal of Hebei University(Natural Science Edition)

基　　金：教育部高等学校博士学科点专项科研基金资助项目(20093227110010);江苏高校优势学科建设工程项目

摘　　要：建立了微球形氯嘧磺隆分子印迹聚合物的合成方法.优化了影响聚合物吸附性能的参数,对聚合物微球进行了性能表征.在氯嘧磺隆印迹微球的合成方法中,功能单体为甲基丙烯酸,交联剂为乙二醇二甲基丙烯酸酯,溶剂为乙腈,引发剂为偶氮二异丁腈,当其用量分别为0.25,1.5,5mmol(物质的量比1∶6∶20)、30mL和0.30mmol时合成的分子印迹聚合物对氯嘧磺隆有最好的特异性吸附能力.运用Scatchard方程测定了该聚合物的结合能力.结果表明,聚合物中具有两类亲和性吸附位点,高亲和性吸附位点的最大表观吸附量Qmax1为63.46μmol/g,平衡常数Kd1为0.655 7mmol/L;低亲和力吸附位点的最大表观吸附量Qmax2为437.085μmol/g,平衡常数Kd2为11.00mmol/L.聚合物微球在重复使用9次后吸附性能基本无下降.本制备方法具有无需研磨筛分即可得到分子印迹微球的特点,便于作为固相萃取柱和色谱柱的填料使用.A method was developed for the synthesis of chlorimuron-ethyl imprinted microspheres. The compositions of chlorimuron-ethyl imprinted microspheres were optimized, and the performances of the microspheres were investigated. In the synthesis method of chlorimuron-ethyl imprinted microspheres, methacrylic acid as functional monomer, ethylene glycol dimethacrylate as crosslinker, acetonitrile as sol- vent and 2, 2＇-azobisisobutyronitrile as the initiator were 0. 25, 1.5, 5 mmol （the molar ration of 1＂6＂ 20）, 30 mL and 0.30 mmol, the prepared imprinted polymers exhibited the best adsorption capacity for chlorimuron-ethyl. Scatchard equation showed that there were two classes of binding sites in the imprinted polymers. The max apparent binding capacity of high affinity＇s binding site Q r^ax~ was 63. 46 pmol/g, and the dissociation constant Ka was 0. 655 7 mmol/L~ while the max apparent binding capacity of low affinity ＂s binding site Q maxe was 437. 085/1tool/g, and the dissociation constant Kd2 was 11.00 mmol/L. The molec- ularly imprinted polymers could be reused nine times without significant decrease on adsorption properties. The chlorimuron-ethyl imprinted microspheres can be directly used in SPE and chromatographic column as packing.

关 键 词：氯嘧磺隆 分子印迹聚合物 微球 吸附性能 

分 类 号：O657.7[理学—分析化学]