电针委中穴对腰肌损伤家兔CK、VEGF和MVD的影响  

Effects of electroacupuncture at BL40 on CK,VEGF and MVD in rabbits with psoas muscle damage

作　　者：彭博[1] 张莉[1] 陈欢[1] 李富运[1] 卫肖艳[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中国康复》2014年第1期3-7,共5页Chinese Journal of Rehabilitation

基　　金：国家自然科学基金(81141120);高等学校博士学科点专项科研基金(20100013110014)

摘　　要：目的:观察电针委中穴对腰肌损伤模型家兔腰肌磷酸肌酸激酶(CK)、血管内皮生长因子(VEGF)及微血管密度(MVD)表达的影响,从血管再生角度探讨针刺委中穴修复腰肌损伤的可能作用机制。方法:将32只雄性家兔随机分为电针委中穴组(委中组)、电针阿是组(阿是组)、模型组和空白组。委中组、阿是组、模型组均采用腰肌钝挫伤的方法造模,造模后委中组和阿是组分别给予对应穴位的电针治疗。2周后取材进行指标检测。结果:造模2周后,与空白组比较,模型组血清CK及腰肌VEGF和MVD的表达均明显增加(P<0.01),委中组和阿是组血清CK和腰肌MVD的表达较空白组明显增加(P<0.01,0.05),但腰肌VEGF的表达变化差异无统计学意义。与模型组比较,委中组和阿是组CK及腰肌VEGF和MVD的表达均下降(P<0.01),委中组和阿是组的组间比较差异无统计学意义。结论:家兔腰肌损伤2周后,电针委中穴可以降低血清CK的含量,促进腰肌损伤修复,其机制可能与电针调控VEGF表达规律而使其提前发挥其再生血管的作用,缩短微血管再生时间而使微血管密度降低有关。Objective：To study the effect of electroacupuncture （EA） at BIA0 on CK,VEGF and MVD in rabbits with psoas muscle damage,and discuss the possible mechanism of EA at BIA0 repairing the psoas muscle damage from the perspective of angiogenesis. Methods： Thirty-two male rabbits were randomly divided into the EA at BIA0 group （BIA0 group）, EA at Ouch point （OP） group （OP group）, model group and blank group. In model group, BL40 group and OP group, the rabbit psoas muscle damage model was established by psoas muscle contusion, there- after,the BE40 group and OP group were given electroacupuncture treatment. Two weeks later, CK, VEGF and MVD contents were detected. Results： Two weeks after modeling, the serum CK and the expression of VEGF and MVD in the psoas muscle in the model group were significantly increased as compared with the blank group （P〈0. 01）. The serum CK and the expression of MVD in the psoas muscle in the BL40 and OP groups were statistically increased （P〈0. 01,0. 05）,but the expression of VEGF in psoas muscle had no significant change in comaprison to the blank group. As compared with the model group, the serum CK and the expression of VEGF and MVD in the psoas muscle in the BIA0 and OP groups were significantly decreased （P〈0. 01）. There was no statistically signifi- cant difference between the BIA0 group and OP group. Conclusion=Two weeks after psoas muscle injury in rabbits, EA at BIA0 can reduce serum CK levels and promote repair of the psoas muscle damage, probably by down-regula- ting the expression of VEGF to advance its role in angiogenesis, and reducing capillary regeneration time to cut down the expression of MVD.

关 键 词：腰肌损伤 电针 委中 微血管密度 血管内皮生长因子 BL40 

分 类 号：R245[医药卫生—针灸推拿学]