田黄冲剂对急性脑出血模型大鼠相关炎性细胞因子的干预作用  

Intervention Effect of Tianhuang Granule on Inflammatory Cytokines in Rats with Cerebral Hemorrhage

作　　者：杨家玥[1] 廖辉[1] 张艳敏[1] 贾铁壮[1] 谢平安[1] 鞠颖[1] 

机构地区：[1]天津市第四中心医院,天津300140

出　　处：《中国中医急症》2014年第2期216-219,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：天津市中医;中西医结合专项基金课题(11089)

摘　　要：目的通过对脑出血模型大鼠炎性细胞因子(IL-1,IL-6、TNF-α)表达的观察,评价田黄冲剂对脑出血模型大鼠脑损伤的干预作用。方法选用健康雄性Wistar大鼠,随机分为模型组、中药组、西药组、中西药组和盐水组。制作脑缺血大鼠模型,对造模成功大鼠除模型组只常规进食水外,其余各组按人与动物等效剂量,分别给予中药(田黄冲剂)、西药(环磷酰胺)、中西药和生理盐水灌胃;并于大鼠清醒后0 h(即刻)、6 h、24 h、48 h和72 h 5个时间段进行行为学观察和神经功能评分;后断头取脑测定炎性细胞因子IL-1,IL-6和TNF-α。结果盐水组Zea-Longa评分与模型组分值相近;中药组和西药组评分均下降,且分值相当;中西药组评分分值下降的最明显。与模型组0时段比较,6 h、24 h、48 h各治疗组IL-6、IL-1均升高(P<0.05或P<0.01);与盐水组比较,大多治疗组IL-6、IL-1均水平较低(P<0.05或P<0.01)。中西药组TNF指标在48、72 h与模型组相近,与盐水组比较大多治疗组TNF均水平较低(P<0.05或P<0.01)。结论田黄冲剂对脑出血模型大鼠炎性细胞因子具有抑制作用,但起效时间较慢多在6 h以后;田黄冲剂与环磷酰胺在24 h以后各时段对炎性细胞因子的抑制作用相当;两药合用各时段对炎性细胞因子的抑制作用明显优于中药组和西药组。说明田黄冲剂可有效抑制脑出血大鼠炎性细胞因子的表达,进而起到脑保护作用。Objective： To evaluate the effect of Tianhuang Granule on acute cerebral hemorrhage injury based on expression of inflammatory factor in rat model （IL-I, IL-6, TNF-α）. Methods： Healthy male wistar rats were ran- domly divided into model group ,traditional Chinese medicine group, Western medicine group, Chinese and West- em medicine group,and Saline group. Build cerebral hemorrhage rat model. The model group was only given wa- ter regularly and the other groups were given traditional Chinese medicine, western medicine, Chinese and western medicine and saline lavage respectively,equivalent of human dose. Rats＇ behaviors and neurological functions were observed and evaluated after waking 0 h （immediate）,6 h,24 h,48 h and 72 h. Then the brain and deter- mine inflammatory cytokines IL-1 ,IL 6 and TNF-alpha. Results： 1.Zea-Longa score： compared with model group,the score of saline group was similar;scores of traditional Chinese medicine group and Western medicine group decreased while the score of Chinese and Western medicine group decreased most obviously. 2.IL-6, IL-1, TNF data： compared with 0 time,model group,the Chinese medicine group and Western medicine group had sta- tistical significance in 6 h,24 h,and 48 h. IL-6,IL-1 index in western medicine group had no statistical signifi- cance in 72 h. Compared with control group,most treatment groups have statistical significance. TNF index in Chinese and western medicine group in 48h and 72 h had no statistical significance, compared with saline group, most of the treatment group has statistical significance. Conclusion： 1.Tianhuang granule has inhibiting effect on inflammatory cytokines in rats with cerebral hemorrhage,but the onset time is slow,mainly after 6 h. 2.The in- hibiting effects in traditional Chinese medicine group and in western medicine group are almost the same in each period after 24 h. 3.Inflammatory cytokines inhibiting effect in Chinese and western medicine group is superior to that of traditional Chinese medicine group and w

关 键 词：田黄冲剂 脑出血 模型大鼠 炎性细胞因子 

分 类 号：R285.5[医药卫生—中药学]