合作猪SLA-DQA基因第4外显子多态性分析  

Analyzing Polymorphism of Hezuo Swine SLA-DQA Gene Exon 4

作　　者：张国华[1] 马小军[1,2] 吕伟丽[1] 

机构地区：[1]甘肃农业大学动物医学院:兰州730037 [2]甘肃省草食动物生物技术重点实验室,兰州730070

出　　处：《生物技术通报》2014年第2期97-101,共5页Biotechnology Bulletin

基　　金：甘肃省自然科学基金项目(096RJZA005)

摘　　要：合作猪的MHC-DQA基因的适应性变异,其抗原识别区域(即外显子4)通过PCR扩增和随后的单链构象多态性(SSCP)和序列分析,结果显示在439个合作猪个体,SLA-DQA第4外显子检出4个等位基因和6个基因型(AA、BB、DD、AB、AC和AD),其中A等位基因和AA基因型的频率最高,为优势基因和优势基因型。对不同型的PCR-SSCP条带测序分析,发现7个突变位点(5 068 bp T→C,5 109 bp和5 149 bp处缺失C,5 131 bp A→G导致丝氨酸变为甘氨酸,5 135 bp C→T,5 234 bp G→A,5 136 bp处插入A)。遗传学分析发现,合作猪多态信息含量(PIC)为0.240 1,属于低度多态,各种基因型的分布不显著。研究结果证实,合作猪SLA—DQA基因第4外显子为低度多态。The Hezuo swine SLA-DQA gene adaptive mutation, antigen recognition region（exon 4）by PCR amplification and subsequent single-strand conformation polymorphism（SSCP）and sequence analysis. The results show that 439 Hezuo swine individual SLA-DQA exon 4 detection of three alleles and six genotypes（AA, BB, CC, AB, AC, BC）the highest frequency of the A allele and AA genotype, genotype was the dominant gene and advantages.On a different type of PCR-SSCP pieces with sequencing analysis found that the fourmutated sites（5 068 bp T → C, 5 109 bp and 5 149 bp insert C, 5 131 bp A → G cause serine into glycine, 5 135 bp C → T, at 5 136 bp insert A, the 5 234 bp G → A lead tyrosyl acid becomes cysteine.Genetic analysis ：cooperation pig polymorphic information content（PIC）was 0.240 1, belongs to the moderate polymorphism;significant distribution of various genotypes.The findings confirm that cooperation pig SLA- DQA gene the 4 exon has low rich genetic polymorphism.

关 键 词：合作猪 SLA-DQA基因 多态性 遗传特性 

分 类 号：S828[农业科学—畜牧学]