肠内喂养温度对大鼠肠缺血-再灌注损伤的影响  

Effects of enteral feeding temperature on intestinal ischemia - reperfusion injury in rats

作　　者：姚平波[1] 赵红[2] 戴利[2] 张平[2] 

机构地区：[1]南华大学附属南华医院重症监护室,湖南衡阳421002 [2]南华大学护理学院,湖南衡阳421001

出　　处：《中国急救医学》2014年第3期259-262,共4页Chinese Journal of Critical Care Medicine

摘　　要：目的探讨肠内喂养温度对肠缺血-再灌注损伤（ischemia—reperfusion injury）大鼠的影响及作用机制。方法将60只雄性SD大鼠随机分为正常对照组、缺血-再灌注组、肠内喂养温度组（10℃、24℃、32℃、40qC组），复制大鼠肠缺血一再灌注损伤模型，观察肠黏膜形态行Chiu评分；采用比色法测定胃黏膜丙二醛（MDA）含量、超氧化物歧化酶（SOD）活力；RT—PCR测定TNF—αmRNA在小肠组织中的表达；光镜下观察肠黏膜病理形态的改变，TUNEL法测定肠上皮细胞凋亡。结果缺血-再灌注损伤组肠黏膜屏障指标Chiu评分、MDA含量、凋亡指数、TNF-αmRNA表达及SOD活力与正常对照组比较差异有统计学意义（P〈0．05）；10℃组Chiu评分、MDA含量、TNF-α．mRNA表达均低于其他各组，SOD活力高于其他各组；而40℃组Chiu评分、MDA含量、TNF-α mRNA表达均高于其他各组，SOD活力低于其他各组。结论降低肠内喂养温度对缺血-再灌注大鼠肠黏膜损伤具有保护作用，其机制可能与低温抗氧化及抑制机体的炎症反应及凋亡有关。Objective To investigate the effects of enteral feeding temperature on intestinal ischemia - reperfusion （I/R） injury in rats and explore its underlying mechanism. Methods A total of sixty male Sprague - Dawley （SD） rats were randomly assigned to six groups with normal control group, I/R group and enteral feeding temperature groups which include 10 ℃ group, 24℃ group, 32℃ group and 40℃ group. Rats with intestinal I/R injury were produced by clamping the superior mesenteric, intestinal mucosal injury index was counted grossly, pathological score of intestinal mucosal injury was evaluated by a light microscope, apoptosis of intestinal epithelial cells was detected by TUNEL, malonaldehyde （MDA） content and superoxide dismutase （SOD） activity of intestinal mucosal were measured by colorimetric method, and the expression of tumor necrosis factor - α （ TNF - α ） of intestinal mucosal cell was examined by RT - PCR. Results Chiu＇s index, apoptotic index, MDA, SOD and TNF - α mRNA of I/R group rats were significantly changed compared to those of normal control group. Compared with other enteral feeding temperature groups, the values of Chiu＇s index, pathological score, MDA content and the amount of positive expression of TNF -α mRNA in 10 ℃ group were decreased. Meanwhile, the SOD activity was increased. However, all those indicators in 40℃ group showed completely opposite results in contrast to 10℃ group. Conclusion Low temperature enteral feeding may protect murine intestinal tissue against I/R injury. And the mechanisms may be due to its actions of preventing oxygen stress, inflammatory responses and apoptosis.

关 键 词：肠内喂养 温度 肠缺血-再灌注损伤 

分 类 号：R656.6[医药卫生—外科学]