RP-HPLC法测定大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷的含量  

RP-HPLC determination of daidzein, genistein, daidzin and genistin in soybean extract

作　　者：张立 

机构地区：[1]西安天诚医药生物工程有限公司,陕西西安710075

出　　处：《中草药》2001年第2期118-120,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的　建立 RP- HPL C法分离检测大豆提取物中大豆苷元、染料木素、大豆苷、染料木苷 4成分含量的方法。方法　用 Zorbax SB- C1 8色谱柱 ,以甲醇 -乙腈 - 0 .1%磷酸 (30∶ 8∶ 6 2 )和甲醇 -乙腈 - 0 .1%磷酸 (16∶ 2 4∶ 6 0 )为流动相分别测定大豆苷元、染料木素和大豆苷、染料木苷 ,检测波长为 2 5 4nm。结果　大豆苷元、染料木素、大豆苷、染料木苷 4成分的平均回收率分别为 98.7% ,99.5 % ,98.4% ,98.6 % ;RSD分别为 1.4% ,0 .9% ,0 .9% ,1.7%(n=4)。结论　方法简便、准确。Object A method for the determination of daidzein, genistein, daidzin and genistin in soybean extract by RP HPLC was established Methods Zorbax SB C 18 column (4 6×15 mm, 5 μm) was used Daidzein and genistein were separated with mobile phase Ⅰ∶methanol acetonitrile 0 1% phosphoric acid solution (30∶8∶62) Daidzin and genistin were separated with mobile phase Ⅱ∶methanol acetonitrie 0 1% phosphoric acid solution (16∶24∶60) The UV detection wavelength was 254 nm Results The average recoveries of daidzein, genistein, daidzin and genistin were 98 7%, 99 5%, 98 4%, 98 6% and the precision (RSD) were 1 4%, 0 9%, 0 9%, 1 7% (n=4) respectively Conclusion: The method was accurate, rapid and with good reproducibility

关 键 词：大豆苷元 染料木素 大豆苷 染料木苷 RP-HPLC 

分 类 号：R286.0[医药卫生—中药学]