吸烟孕妇的胎儿CYP1A1、GST基因多态性与胎儿先天性畸形易感性的关系  

Relationship between gene polymorphisms of CYP1A1,GST in neonates of smoking pregnant women and susceptibility to congenital malformation

作　　者：王宏宇[1] 王晨虹[1] 胡端林 

机构地区：[1]南方医科大学附属深圳市妇幼保健院,广东深圳518028 [2]广州医科大学,518028

出　　处：《中国妇幼保健》2014年第8期1232-1235,共4页Maternal and Child Health Care of China

摘　　要：目的：探讨被动吸烟孕妇的胎儿GSTM1、GSTF1基因及细胞色素P4501A1（CYP1A1）基因多态性对胎儿先天性畸形的影响。方法：采用病例一对照研究方法，选取2012年4月～2013年4月在深圳市妇幼保健院产科入院分娩单胎、活产的87例畸形儿为研究对象，将其设为病例组，选择同期出生的181例正常新生儿作为对照组；应用多重PCR技术确定先天性畸形儿和正常新生儿GSTM1、GSTT1基因型，采用限制性内切酶PCR（RFLP—PCR）技术确定先天性畸形儿和正常新生儿CYP1A1基因型。结果：共调查268例新生儿，其中病例组87例，对照组181例。病例组的CYP1A1基因MSP1多态突变型的频率与对照组比较差异有统计学意义（P〈0．05），携带突变型CYP1A1基因的个体为先天性畸形的危险度比非突变型（杂合型和野生型）个体高；单一的GSTM1和GSTF1基因型分布频率在病例组与对照组中比较差异无统计学意义（P〉0．05）；同时携带CYP1A1突变型和GSTMI、GSTT1缺失型的联合基因型个体为先天性畸形儿的风险明显增加。结论：CYP1A1基因MSP1多态突变型可能与先天性畸形儿的易感性有关；单一的GSTM1和GSTr1基因缺失型可能与先天性畸形儿的易感性无关；同时携带CYP1A1突变型和GSTM1、GSTT1缺失型的联合基因型可能是胎儿先天性畸形的易感因素之一。Abstract） Objective： To explore the effects of gene polymorphisms of CYP4501A1, GSTM1 and GSTT1 on neonatal congenital malformation of pregnant women passive smoking. Methods： A case - control study was conducted to investigate the neonatal congenital mal- formation of single birth and live birth, whose mother had been passive smoking, in obstetric department of the Shenzhen Maternity and Child Heahhcare Hospital from April 2012 to April 2013, the neonates with normal birth weight who were born during the same period were selected as control group; PCR and RELP -PCR technique was used to determine the genotypes of GSTI＇I, GSTM1, and CYP4501A1 of neonates in the two groups. Results： A total of 268 neonates were obtained, including 87 in congenital malformation group and 181 in nor- mal control group. There was statistically significant difference in the frequency of MSP1 homozygous mutant of CYP1A1 gene between neo- natal congenital malformation group and normal neonatal control group （ P 〈 0. 05 ） . The risk degree of congenital malformation among the neonates carrying mutant type was significantly higher than those among the neonates nonmutant type （ heterozygous mutant and wild type） ; there was no statistically significant difference in the distribution frequency of deletion type of single GSTM1 gene and GSTI＇I gene between neonatal congenital malformation group and normal control group （ P 〉 0.05 ） ; the risk of neonatal congenital malformation increased signif- icantly in the neonates carrying mutant type of CYP1 A1 gene and deletion types of GSTM1 gene and GSTI＇I gene. Conclusion： MSP1 homo- zygous mutant of CYP1A1 gene may be correlated with susceptibility of neonatal congenital malformation. Deletion type of single GSTM1 gene or GSTT1 gene may be not related to susceptibility of neonatal congenital malformation. Carrying homozygous mutant type of CYP1A1 gene and deletion types of GSTM1 gene and GSTFI gene may be one of the susceptible factors of neonatal congenital malforma

关 键 词：胎儿先天性畸形 被动吸烟 CYP1A1 GST 基因多态性 

分 类 号：R722[医药卫生—儿科]