生长因子对山羊无腔卵泡卵母细胞体外生长的影响  

EFFECTS OF GROWTH FACTORS ON THE IN VITRO GROWTH OF HIRCINE NON ANTRAL FOLLICLE OOCYTES

作　　者：周欢敏[1] 张涌[2] 

机构地区：[1]内蒙古农牧学院动科系,呼和浩特010018 [2]西北农业大学发育生物学研究室,杨凌712100

出　　处：《畜牧兽医学报》2001年第1期23-27,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：农业部生物技术重点项目! (95牧- 0 1- 0 4- 0 1)

摘　　要：山羊无腔卵泡卵母细胞在胰岛素样生长因子I(IGF I)和表皮生长因子 (EGF)及碱性成纤维细胞生长因子 (bFGF)单独或联合存在时 ,与FSH一道体外培养 9d ,结果表明 ,IGF I( 10 0ng/mL)能有效地维持卵泡卵母细胞的存活并促进其生长 ,存活率达 75 0 0 % ,相对生长率达 4 5 0 6% ;EGF( 50ng/mL)能提高卵泡卵母细胞的体外存活率 ,类似于IGF I ,但对其生长相对表现出抑制影响 ;bFGF( 50ng/mL)对卵泡卵母细胞的体外存活具有促进作用 ,但对卵母细胞生长的促进作用不明显 (卵母细胞的存活率为 76 92 % ,相对生长率为 31 55% )。IGF I( 10 0ng/mL)和EGF( 50ng/mL)联合存在时 ,不论是对卵泡卵母细胞的存活 ,还是对生长均产生了最好的效果 ,卵母细胞存活率达到 90 91% ,相对生长达 4 8 2 4 %。本试验结果一个共同点是在FSH存在的情况下 ,这 3种生长因子单独或有选择的联合 。WT5BZ]In the presence of FSH,the hircine non antral follicle oocytes were cultured for 9 days with IGF I,EGF and bFGF alone or combinedly.The results showed that IGF I(100ng/mL)effectively maitained the survival of follicle oocytes and promoted their growth,and the survival rate and the relative growth rate of oocytes were 75 00% and 45 06% respectively.EGF(50ng/mL)enhanced the survival rate of the oocytes like IGF I,but had a negative effect on the growth of oocytes.bFGF(50ng/mL)had a promoting effect on the survival in vitro of oocytes but not obvious on the growth of them (the survival rate and the relative growth rate of oocytes were 76 92% and 31 55% respectively).IGF I(100ng/mL) and EGF(50ng/mL)combination had the highest effects on both of the survival and the growth of oocytes.The survival rate and the relative growth rate of oocytes were 90 91% and 48 24% respectively.It has been shown that in the presence of FSH these kinds of growth factors alone or choosingly combinedly gave a positive regulation to the survival in vitro of hircine non antral follicle oocytes.

关 键 词：山羊 无腔卵泡 卵母细胞 体外生长 存活率 胰岛素样生长因子 表皮生长因子 碱性成纤维细胞生长因子 

分 类 号：S827.1[农业科学—畜牧学] S814.8[农业科学—畜牧兽医]